Clostridium difficile binary toxin (CDT) and diarrhea

被引：39

作者：

Carman, Robert J. ^{[1
]}

Stevens, Adam L. ^{[1
]}

Lyerly, Matthew W. ^{[1
]}

Hiltonsmith, Megan F. ^{[1
]}

Stiles, Bradley G. ^{[2
,3
]}

Wilkins, Tracy D. ^{[1
]}

机构：

[1] TechLab Inc, Blacksburg, VA 24060 USA

[2] USA, Med Res Inst Infect Dis, Dept Immunol & Mol Biol, Toxinol Div, Ft Detrick, MD 21702 USA

[3] Wilson Coll, Chambersburg, PA 17201 USA

来源：

ANAEROBE | 2011年 / 17卷 / 04期

关键词：

Clostridium difficile; CDT; Binary toxin; Feces; Ribotype; PERFRINGENS IOTA-TOXIN; ADP-RIBOSYLTRANSFERASE; GENE; STRAINS; DISEASE; LOCUS;

D O I：

10.1016/j.anaerobe.2011.02.005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clostridium difficile is a major enteropathogen of humans. It produces two main virulence factors, toxins A and B. A third, less well known toxin, C. difficile toxin (CDT), is a binary toxin composed of distinct enzymatic (CdtA) and cell binding/translocation (CdtB) proteins. We used a novel enzyme linked immunoassay (EIA) to detect CdtB protein in feces and culture fluids. Additionally. PCR was used to assay C. difficile isolates from fecal samples for the CDT locus (CdtLoc). Although the results from 80 isolates suggest no relationship between toxin concentrations in situ and in vitro, there is a good correlation between PCR detection of the cdtB gene and EIA detection of CdtB protein in vitro. Possible implications of the detection of CDT in patients are discussed. (C) 2011 Elsevier Ltd. All rights reserved.

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页码：161 / 165

页数：5

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