Complete genome sequence of Ruminococcaceae bacterium CPB6: A newly isolated culture for efficient n-caproic acid production from lactate

被引:48
作者
Tao, Yong [1 ,2 ]
Zhu, Xiaoyu [1 ,2 ]
Wang, Han [1 ,2 ]
Wang, Yi [3 ]
Li, Xiangzhen [1 ,2 ]
Jin, Hong [4 ]
Rui, Junpeng [1 ,2 ]
机构
[1] Chinese Acad Sci, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China
[2] Chinese Acad Sci, Chengdu Inst Biol, Environm Microbiol Key Lab Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China
[3] Auburn Univ, Dept Biosyst Engn, Auburn, AL 36849 USA
[4] Chengdu Med Coll, Chengdu 610083, Sichuan, Peoples R China
关键词
Genome; Ruminococcaceae bacterium; n-Caproic acid; Chain elongation; Electron donor; MEDIUM-CHAIN CARBOXYLATES; MEGASPHAERA-ELSDENII; HEXANOIC ACID; EXTRACTIVE FERMENTATION; CLOSTRIDIUM KLUYVERI; REACTOR MICROBIOMES; ELONGATION; METABOLISM; ETHANOL; BUTYRATE;
D O I
10.1016/j.jbiotec.2017.07.036
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
n-caproic acid (CA) is a valuable chemical feedstock for various industrial applications. Biological production of CA from renewable carbon sources has attracted a lot of attentions recently. We lately reported the new culture Ruminococcaceae bacterium CPB6, which was isolated from a microbiome for efficient CA production from lactate. To further elucidate its metabolism, we sequenced the whole genome of the strain. The size of the complete genome is 2,069,994 bp with 50.58% GC content; no plasmid was identified. Sets of genes involved in the fatty acid biosynthesis via acyl carrier protein (ACP) and coenzyme A (CoA) as well as lactate oxidation/reduction pathways were identified in the genome. These genes were inferred to be correlated with the CA production. The complete genome sequence provides essential information for the elucidation of the metabolism for CA production from lactate, and further improvement of the strain through genetic engineering for enhanced CA production and other biotechnological purposes.
引用
收藏
页码:91 / 94
页数:4
相关论文
共 28 条
[1]   Chain elongation with reactor microbiomes: upgrading dilute ethanol to medium-chain carboxylates [J].
Agler, Matthew T. ;
Spirito, Catherine M. ;
Usack, Joseph G. ;
Werner, Jeffrey J. ;
Angenent, Largus T. .
ENERGY & ENVIRONMENTAL SCIENCE, 2012, 5 (08) :8189-8192
[2]   Induction of Colonic Regulatory T Cells by Indigenous Clostridium Species [J].
Atarashi, Koji ;
Tanoue, Takeshi ;
Shima, Tatsuichiro ;
Imaoka, Akemi ;
Kuwahara, Tomomi ;
Momose, Yoshika ;
Cheng, Genhong ;
Yamasaki, Sho ;
Saito, Takashi ;
Ohba, Yusuke ;
Taniguchi, Tadatsugu ;
Takeda, Kiyoshi ;
Hori, Shohei ;
Ivanov, Ivaylo I. ;
Umesaki, Yoshinori ;
Itoh, Kikuji ;
Honda, Kenya .
SCIENCE, 2011, 331 (6015) :337-341
[3]   Clostridium kluyverii, an organism concerned in the formation of caproic acid from ethyl alcohol [J].
Barker, HA ;
Taha, SM .
JOURNAL OF BACTERIOLOGY, 1942, 43 (03) :347-363
[4]   THE SYNTHESIS OF BUTYRIC AND CAPROIC ACIDS FROM ETHANOL AND ACETIC ACID BY CLOSTRIDIUM KLUYVERI [J].
BARKER, HA ;
KAMEN, MD ;
BORNSTEIN, BT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1945, 31 (12) :373-381
[5]   IMG/M: integrated genome and metagenome comparative data analysis system [J].
Chen, I-Min A. ;
Markowitz, Victor M. ;
Chu, Ken ;
Palaniappan, Krishna ;
Szeto, Ernest ;
Pillay, Manoj ;
Ratner, Anna ;
Huang, Jinghua ;
Andersen, Evan ;
Huntemann, Marcel ;
Varghese, Neha ;
Hadjithomas, Michalis ;
Tennessen, Kristin ;
Nielsen, Torben ;
Ivanova, Natalia N. ;
Kyrpides, Nikos C. .
NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D507-D516
[6]  
Chin CS, 2013, NAT METHODS, V10, P563, DOI [10.1038/nmeth.2474, 10.1038/NMETH.2474]
[7]   In Situ Biphasic Extractive Fermentation for Hexanoic Acid Production from Sucrose by Megasphaera elsdenii NCIMB 702410 [J].
Choi, Kieun ;
Jeon, Byoung Seung ;
Kim, Byung-Chun ;
Oh, Min-Kyu ;
Um, Youngsoon ;
Sang, Byoung-In .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 171 (05) :1094-1107
[8]  
Desbois Andrew P, 2012, Recent Pat Antiinfect Drug Discov, V7, P111
[9]   Promoting chain elongation in mixed culture acidification reactors by addition of ethanol [J].
Grootscholten, T. I. M. ;
dal Borgo, F. Kinsky ;
Hamelers, H. V. M. ;
Buisman, C. J. N. .
BIOMASS & BIOENERGY, 2013, 48 :10-16
[10]   1-Hexene: a renewable C6 platform for full-performance jet and diesel fuels [J].
Harvey, Benjamin G. ;
Meylemans, Heather A. .
GREEN CHEMISTRY, 2014, 16 (02) :770-776