NF-κB activation in Kupffer cells after partial hepatectomy

The transcription factor nuclear factor-kappa B (NF-kappa B) is activated during liver regeneration after partial hepatectomy. However, the physiological role and cellular localization of NF-kappa B activation are unresolved. In this study, we used an adenoviral vector expressing a mutated form of IkB alpha to inhibit NF-kappa B activity during liver regeneration. After partial hepatectomy in mice, introduction of Ad5I kappa B, but not a control virus (Ad5GFP), resulted in increased liver injury and decreased hepatocyte proliferation. Hepatocyte apoptosis was not observed. To investigate the kinetics and cellular localization of NF-kappa B-induced transcription during liver regeneration, we generated a transgenic mouse expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-kappa B cis elements (cis-NF-kappa B-EGFP). During liver regeneration, EGFP expression was detected within 12 h and was primarily located in Kupffer cells. Our data demonstrate that activation of NF-kappa B initially occurs in Kupffer cells after partial hepatectomy in mice.