Alpha-Amylase Production by Bacillus subtilis RSKK96 in Submerged Cultivation

被引：0
作者：
Akcan, Nurullah [1 ]
Uyar, Fikret [2 ]
Guven, Aysel [3 ]
机构：
[1] Siirt Univ, Hlth High Sch, TR-56100 Siirt, Turkey
[2] Dicle Univ, Dept Biol, Fac Sci, TR-21280 Diyarbakir, Turkey
[3] Kafkas Univ, Fac Sci Art, Dept Biol, TR-36100 Kars, Turkey
来源：
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI | 2011年 / 17卷
关键词：
Bacillus subtilis RSKK96; alpha-Amylase; Submerged fermentation; Enzyme production;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The bacterial strain Bacillus subtilis RSKK96 was shown to produce extracellular alpha-amylase. Enzyme synthesis occurred at 72 h with an optimum of 37 degrees C. Effects of various carbon, nitrogen, amino acid sources and chemicals on alpha-amylase production were examined. FeSO4, ZnSO4 and CuSO4 inhibited bacterial growth as a result, amylase production. Maximum alpha-amylase production (858.6 +/- 41.9 U/mg) was obtained in a medium containing 0.5% cotton stalk in 72 h.
引用
收藏
页码：S17 / S22
页数：6
相关论文
共 23 条
[1]  
Albayrak Nedim, 1996, Turkish Journal of Biology, V20, P47
[2]  
ALYER PVD, 2004, AFR J BIOTECHNOL, V3, P519
[3]   A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing [J].
Asgher, M. ;
Asad, M. Javaid ;
Rahman, S. U. ;
Legge, R. L. .
JOURNAL OF FOOD ENGINEERING, 2007, 79 (03) :950-955
[4]   AMYLASES, ALPHA AND BETA [J].
BERNFELD, P .
METHODS IN ENZYMOLOGY, 1955, 1 :149-158
[5]   Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp isolate ANT-6 [J].
Burhan, A ;
Nisa, U ;
Gökhan, C ;
Ömer, C ;
Ashabil, A ;
Osman, G .
PROCESS BIOCHEMISTRY, 2003, 38 (10) :1397-1403
[6]   EFFECTS OF PHYTIC ACID, DIVALENT-CATIONS, AND THEIR INTERACTIONS ON ALPHA-AMYLASE ACTIVITY [J].
DESHPANDE, SS ;
CHERYAN, M .
JOURNAL OF FOOD SCIENCE, 1984, 49 (02) :516-&
[7]   Biochemical Characterization of Raw-starch-digesting Alpha Amylase Purified from Bacillus amyloliquefaciens [J].
Gangadharan, Dhanya ;
Nampoothiri, K. Madhavan ;
Sivaramakrishnan, Swetha ;
Pandey, Ashok .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 158 (03) :653-662
[8]   Characterization and stability of proteases from Penicillium sp produced by solid-state fermentation [J].
Germano, S ;
Pandey, A ;
Osaku, CA ;
Rocha, SN ;
Soccol, CR .
ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (02) :246-251
[9]   Microbial α-amylases:: a biotechnological perspective [J].
Gupta, R ;
Gigras, P ;
Mohapatra, H ;
Goswami, VK ;
Chauhan, B .
PROCESS BIOCHEMISTRY, 2003, 38 (11) :1599-1616
[10]  
Kiran Ozlem, 2005, Turkish Journal of Biology, V29, P99
123