Mass spectrometry informs the structure and dynamics of membrane proteins involved in lipid and drug transport

被引：12
作者：
Bolla, Jani R. [1 ,2 ]
Fiorentino, Francesco [1 ,2 ]
V. Robinson, Carol [1 ,2 ]
机构：
[1] Univ Oxford, Dept Chem, Phys & Theoret Chem Lab, Oxford OX1 3QZ, England
[2] Kavli Inst Nanosci Discovery, 3 South Parks Rd, Oxford OX1 3QU, England
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2021年 / 70卷
基金：
英国医学研究理事会;
关键词：
OUTER-MEMBRANE; LIPOPOLYSACCHARIDE TRANSPORT; EFFLUX PUMPS; CRYO-EM; RESISTANCE; TRANSLOCATION; IDENTIFY; MSBA;
D O I：
10.1016/j.sbi.2021.03.014
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Membrane proteins are important macromolecules that play crucial roles in many cellular and physiological processes. Over the past two decades, the use of mass spectrometry as a biophysical tool to characterise membrane proteins has grown steadily. By capturing these dynamic complexes in the gas phase, many unknown small molecule interactions have been revealed. One particular application of this research has been the focus on antibiotic resistance with considerable efforts being made to understand underlying mechanisms. Here we review recent advances in the application of mass spectrometry that have yielded both structural and dynamic information on the interactions of antibiotics with proteins involved in bacterial cell envelope biogenesis and drug efflux.
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收藏
页码：53 / 60
页数：8
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