15N-LABELING EXPERIMENTS TO DISSECT THE CONTRIBUTIONS OF HETEROTROPHIC DENITRIFICATION AND ANAMMOX TO NITROGEN REMOVAL IN THE OMZ WATERS OF THE OCEAN

被引：0

作者：

Holtappels, Moritz ^{[1
]}

Lavik, Gaute ^{[1
]}

Jensen, Marlene M. ^{[2
]}

Kuypers, Marcel M. M. ^{[1
,3
]}

机构：

[1] Max Planck Inst Marine Microbiol, Bremen, Germany

[2] Univ So Denmark, Inst Biol, Copenhagen, Denmark

[3] Univ So Denmark, Nord Ctr Earth Evolut NordCEE, Copenhagen, Denmark

来源：

METHODS IN ENZYMOLOGY: RESEARCH ON NITRIFICATION AND RELATED PROCESSES, VOL 486, PART A | 2011年 / 486卷

关键词：

ANAEROBIC AMMONIUM OXIDATION; OXYGEN-MINIMUM ZONES; BLACK-SEA; NITRATE REDUCTION; UPWELLING SYSTEM; MARINE-SEDIMENTS; BALTIC SEA; BACTERIA; INHIBITORS; NITRIFICATION;

D O I：

10.1016/S0076-6879(11)86010-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, N-15-labeling experiments have become a powerful tool investigating rates and regulations of microbially mediated nitrogen loss processes in the ocean. This chapter introduces the theoretical and practical aspects of N-15-labeling experiments to dissect the contribution of denitrification and anammox to nitrogen removal in oxygen minimum zones (OMZs). We provide a detailed description of the preparation and realization of the experiments on board. Subsequent measurements of N-2 isotopes using gas chromatography mass spectrometry as well as processing of data and calculation of anammox and denitrification rates are explained. Important supplementary measurements are specified, such as the measurement of nanomolar concentrations of ammonium, nitrite, and nitrate. Nutrient profiles and N-15-experiments from the Peruvian OMZ are presented and discussed as an example.

引用

页码：223 / 251

页数：29

共 39 条

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