The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection

被引:127
作者
Mirsky, ML
Olmstead, CA
Da, Y
Lewin, HA
机构
[1] UNIV ILLINOIS, DEPT ANIM SCI, IMMUNOGENET LAB, EDWARD R MADIGAN LAB 206, URBANA, IL 61801 USA
[2] UNIV ILLINOIS, DEPT VET PATHOBIOL, URBANA, IL 61801 USA
关键词
D O I
10.1128/JVI.70.4.2178-2183.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The bovine leukemia virus (BLV) is an oncogenic retrovirus that is associated with the development of persistent lymphocytosis (PL) and lymphoma in cattle. While B lymphocytes have been shown to be the primary cellular target of BLV, recent studies suggest that some T lymphocytes and monocytes may be infected by the virus. Because virally altered functions of monocytes and/or T cells could contribute to the development of lymphoproliferative disease, we sought to clarify the distribution of the BLV provirus in subpopulations of peripheral blood mononuclear cells in seropositive cows with and without PL. CD2(+) T cells, monocytes, and CD5(+) and CD5(-) B cells were sorted by flow cytometry and tested for the presence of BLV by single-cell PCR. We did not obtain convincing evidence that peripheral blood monocytes or T lymphocytes contain the BLV provirus in seropositive cows with or without PL. In seropositive cows without PL (n = 14), BLV-infected CD5(+) and CD5(-) B cells accounted for 9.2% +/- 19% and 0.1% +/- 1.8% of circulating B lymphocytes, respectively. In cows with PL (n = 5), BLV-infected CD5(+) and CD5(-) B cells accounted for 66% +/- 4.8% and 13.9% +/- 6.6% of circulating B lymphocytes, respectively. The increase in lymphocyte numbers in cows with PL was entirely attributable to the 45-fold and 99-fold expansions of infected CD5(+) and CD5(-) B-cell populations, respectively. Our results demonstrate that B cells are the only mononuclear cells in peripheral blood that are significantly infected with BLV. On the basis of the absolute numbers of infected cells in seropositive, hematologically normal animals, there appear to be differences in susceptibility to viral spread in vivo that may be under the genetic control of the host.
引用
收藏
页码:2178 / 2183
页数:6
相关论文
共 57 条
[1]   STUDIES ON DEVELOPMENT OF PERSISTENT LYMPHOCYTOSIS AND INFECTION WITH BOVINE C-TYPE LEUKEMIA-VIRUS (BLV) IN CATTLE [J].
ABT, DA ;
MARSHAK, RR ;
FERRER, JF ;
PIPER, CE ;
BHATT, DM .
VETERINARY MICROBIOLOGY, 1976, 1 (2-3) :287-300
[2]  
BUEHRING GC, 1994, LAB INVEST, V71, P359
[3]  
BURNY A, 1988, ADV VET SCI COMP MED, V32, P149
[4]  
CANN AJ, 1990, VIROLOGY, P1501
[5]   PEDIGREE STUDIES IN BOVINE LYMPHOSARCOMA [J].
CROSHAW, JE ;
ABT, DA ;
IPSEN, J ;
MARSHAK, RR ;
HARE, WCD ;
SWITZER, J ;
DUTCHER, RM .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1963, 108 (03) :1193-&
[6]  
Davis W. C., 1990, Monographs in Animal Immunology, V1, P47
[7]   BOVINE LEUKEMIA-VIRUS (BLV)-INFECTED B-CELLS EXPRESS A MARKER SIMILAR TO THE CD5 T-CELL MARKER [J].
DEPELCHIN, A ;
LETESSON, JJ ;
LOSTRIETRUSSART, N ;
MAMMERICKX, M ;
PORTETELLE, D ;
BURNY, A .
IMMUNOLOGY LETTERS, 1989, 20 (01) :69-76
[8]   EXPERIMENTS WITH CLONED COMPLETE TUMOR-DERIVED BOVINE LEUKEMIA-VIRUS INFORMATION PROVE THAT THE VIRUS IS TOTALLY EXOGENOUS TO ITS TARGET ANIMAL SPECIES [J].
DESCHAMPS, J ;
KETTMANN, R ;
BURNY, A .
JOURNAL OF VIROLOGY, 1981, 40 (02) :605-609
[9]   MASSIVE COVERT INFECTION OF HELPER T-LYMPHOCYTES AND MACROPHAGES BY HIV DURING THE INCUBATION PERIOD OF AIDS [J].
EMBRETSON, J ;
ZUPANCIC, M ;
RIBAS, JL ;
BURKE, A ;
RACZ, P ;
TENNERRACZ, K ;
HAASE, AT .
NATURE, 1993, 362 (6418) :359-362
[10]  
ESTEBAN EN, 1985, CANCER RES, V45, P3225