Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging

被引：116
作者：
Strauss, Sebastian [1 ,2 ,3 ]
Nickels, Philipp C. [1 ,2 ,3 ]
Strauss, Maximilian T. [1 ,2 ,3 ]
Sabinina, Vilma Jimenez [4 ]
Ellenberg, Jan [4 ]
Carter, Jeffrey D. [5 ]
Gupta, Shashi [5 ]
Janjic, Nebojsa [5 ]
Jungmann, Ralf [1 ,2 ,3 ]
机构：
[1] Ludwig Maximilians Univ Munchen, Dept Phys, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Ctr Nanosci, Munich, Germany
[3] Max Planck Inst Biochem, Martinsried, Germany
[4] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany
[5] SomaLogic Inc, Boulder, CO USA
来源：
NATURE METHODS | 2018年 / 15卷 / 09期
基金：
欧洲研究理事会;
关键词：
OPTICAL RECONSTRUCTION MICROSCOPY; SUPERRESOLUTION MICROSCOPY; LOCALIZATION MICROSCOPY; ARTIFACTS; TRACKING; BIOLOGY; LIMIT;
D O I：
10.1038/s41592-018-0105-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.
引用
收藏
页码：685 / +
页数：6
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