Photomodulation of Protein Trans-Splicing Through Backbone Photocaging of the DnaE Split Intein

(Figure Presented) Lightly spliced inteins: A novel strategy to modulate the assembly and trans-splicing activity of the Ssp DnaE split intein was achieved by introducing two photolabile protecting groups onto the backbone of the C-intein polypeptide. This modification was not only able to efficiently block the trans-splicing activity, but also reduce significantly the binding affinity constant between the Cand N-intein fragments. The original activity of the wildtype split intein could be fully recovered by brief exposure to UV light. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.