Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry

被引：225

作者：

Kumar, Ravindra

El-Sagheer, Afaf

Tumpane, John

Lincoln, Per

Wilhelmsson, L. Marcus

Brown, Tom ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Chalmers, Dept Chem & Biol Engn Phys Chem, SE-41296 Gothenburg, Sweden

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 21期

关键词：

D O I：

10.1021/ja070273v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.

引用

页码：6859 / 6864

页数：6