Antibacterial and antitumor activity of Bogorol B-JX isolated from Brevibacillus laterosporus JX-5

被引:19
作者
Jiang, Hongxia [1 ,2 ]
Ji, Chao [2 ]
Sui, Junkang [2 ]
Sa, Rongbo [2 ]
Wang, Xiaohui [2 ]
Liu, Xunli [3 ]
Guo, Tai L. [4 ]
机构
[1] Taishan Med Univ, Sch Life Sci, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
[3] Shandong Agr Univ, Coll Forestry, Tai An 271018, Shandong, Peoples R China
[4] Univ Georgia, Coll Vet Med, Dept Vet Biosci & Diagnost Imaging, Athens, GA 30602 USA
关键词
Antimicrobial peptide; Antibacterial; Antitumor; Methicillin-resistant Staphylococcus aureus (MRSA); Bogorol; ANTIMICROBIAL PEPTIDES; NK-LYSIN; CANCER; ANTIBIOTICS; APOPTOSIS; PHOSPHATIDYLSERINE; CELLS; DERIVATIVES; RESISTANCE; MECHANISM;
D O I
10.1007/s11274-017-2337-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Antimicrobial peptides are promising anti-infective agent candidates because they have a broad antimicrobial spectrum and bioactivity and are unlikely to elicit antibiotic resistance. The bogorols represent a new cationic antibiotic peptide and possess great therapeutic potential because of their bioactivity and precise mode of action. Here, we report that Bogorol B-JX (BBJX), a peptide previously isolated from Brevibacillus laterosporus JX-5 by us, has significant antibacterial and antitumor activities in vitro. BBJX was found to inhibit methicillin-resistant Staphylococcus aureus (MRSA) at 2.5 mu g/mL with distinct mechanisms of action from those against Bacillus bombyseptieus and Escherichia coli. It penetrates MRSA membrane with little visible destruction and binds to genomic DNA. BBJX could inhibit the proliferation of human histiocytic lymphoma cell line U-937 and ConA-activated spleen cells at 5 mu g/mL, but was not cytotoxic to the Jurkat cells, resting spleen cells or differentiated macrophage-like U-937 immunocytes. Moreover, BBJX caused apoptosis of U-937 cells by opening the mitochondrial permeability transition pore and stimulating the production of reactive oxygen species. Taken together, these studies provided basis for future medical application of the bogorols.
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页数:11
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