Three small, cryptic plasmids from Aeromonas salmonicida subsp salmonicida A449

被引：49

作者：

Boyd, J ^{[1
]}

Williams, J ^{[1
]}

Curtis, B ^{[1
]}

Kozera, C ^{[1
]}

Singh, R ^{[1
]}

Reith, M ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Marine Biosci, Halifax, NS B3H 3Z1, Canada

来源：

PLASMID | 2003年 / 50卷 / 02期

关键词：

furunculosis; plasmid addiction; ColE1-type replicon; CoIE2-type replicon;

D O I：

10.1016/S0147-619X(03)00058-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide sequences of three small (5.2-5.6 kb) plasmids from Aeromonas salmonicida subsp. salmonicida A449 are described. Two of the plasmids (pAsa1 and pAsa3) use a ColE2-type replication mechanism while the third (pAsa2) is a ColE1-type replicon. Insertions in the Rep protein and oriV region of the ColE2-type plasmids provide subtle differences that allow them to be maintained compatibly. All three plasmids carry genes for mobilization (mobABCD), but transfer genes are absent and are presumably provided in trans. Two of the plasmids, pAsa1 and pAsa3, carry toxin-antitoxin gene pairs, most probably to ensure plasmid stability. One open reading frame (ORF), orf1, is conserved in all three plasmids, while other ORF's are plasmid-specific. A survey of A. salmonicida strains indicates that pAsa1 and pAsa2 are present in all 12 strains investigated, while pAsa3 is present in I I and a fourth plasmid, pAsa11. is present in 7. Crown Copyright (C) 2003 Published by Elsevier Inc. All rights reserved.

引用

页码：131 / 144

页数：14