Landscape of the SOX2 protein-protein interactome

被引：56

作者：

Fang, Xuefeng ^{[1
,2
,3
,4
]}

Yoon, Jae-Geun ^{[1
]}

Li, Lisha ^{[2
]}

Tsai, Yihsuan S. ^{[3
]}

Zheng, Shu ^{[4
]}

Hood, Leroy ^{[5
]}

Goodlett, David R. ^{[3
]}

Foltz, Gregory ^{[1
]}

Lin, Biaoyang ^{[1
]}

机构：

[1] Swedish Med Ctr, Swedish Neurosci Inst, Seattle, WA 98122 USA

[2] Zhejiang Univ, Zhejiang California Int NanoSyst Inst, Hangzhou 310003, Zhejiang, Peoples R China

[3] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA

[4] Zhejiang Univ, Sch Med, Affiliated Hosp 2,China Natl Minist Educ, Inst Canc,Key Lab Canc Prevent & Intervent, Hangzhou 310003, Zhejiang, Peoples R China

[5] Inst Syst Biol, Seattle, WA USA

来源：

PROTEOMICS | 2011年 / 11卷 / 05期

关键词：

Biomedicine; Glioblastoma multiforme; Heterogeneous nuclear ribonucleoprotein; Mass spectrometry; SOX2; PLURIPOTENT STEM-CELLS; RNA-BINDING PROTEIN; HNRNP-K-PROTEIN; TRANSCRIPTION FACTORS; INITIATING CELLS; DNA; PROLIFERATION; GLIOBLASTOMA; OCT4; FIBROBLASTS;

D O I：

10.1002/pmic.201000419

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

SOX2 is a key gene implicated in maintaining the stemness of embryonic and adult stem cells that appears to re-activate in several human cancers including glioblastoma multiforme. Using immunoprecipitation (IP)/MS/MS, we identified 144 proteins that are putative SOX2 interacting proteins. Of note, SOX2 was found to interact with several heterogeneous nuclear ribonucleoprotein family proteins, including HNRNPA2B1, HNRNPA3, HNRNPC, HNRNPK, HNRNPL, HNRNPM, HNRNPR, HNRNPU, as well as other ribonucleoproteins, DNA repair proteins and helicases. Gene ontology (GO) analysis revealed that the SOX2 interactome was enriched for GO terms GO:0030529 ribonucleoprotein complex and GO:0004386 helicase activity. These findings indicate that SOX2 associates with the heterogeneous nuclear ribonucleoprotein complex, suggesting a possible role for SOX2 in post-transcriptional regulation in addition to its function as a transcription factor.

引用

页码：921 / 934

页数：14

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