Metabolomic and Transcriptomic Analysis of Flavonoid Biosynthesis in Two Main Cultivars of Actinidia arguta Sieb.Zucc. Grown in Northern China

被引：4

作者：

Wang, Yubo ^{[1
]}

Wang, Yong ^{[2
]}

Sun, Jun ^{[3
]}

Dai, Yue ^{[4
]}

Yang, Fengyan ^{[5
]}

Jiang, Hui ^{[2
]}

Irfan, Muhammad ^{[6
]}

Chen, Lijing ^{[1
]}

机构：

[1] Shenyang Agr Univ, Coll Biosci & Biotechnol, Key Lab Agr Biotechnol, Shenyang, Peoples R China

[2] Univ Sci & Technol Liaoning, Coll Chem Engn, Anshan, Peoples R China

[3] Liaoning Inst Econ Forestry, Dalian, Peoples R China

[4] Shandong Xianda Agrochem Co Ltd, Jinan, Peoples R China

[5] Shenyang Acad Agr Sci, Shenyang Modern Agr R&D Serv Ctr, Shenyang, Peoples R China

[6] Univ Sargodha, Dept Biotechnol, Sargodha, Pakistan

来源：

FRONTIERS IN PLANT SCIENCE | 2022年 / 13卷

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Actinidia arguta Sieb; Zucc; flavonoids; biosynthesis; widely targeted metabolome; transcriptome; joint analysis; TARTARY BUCKWHEAT; ANTHOCYANIN BIOSYNTHESIS; KIWIFRUIT; EXPRESSION; GENE; IDENTIFICATION; CLONING; ACCUMULATION; ANTIOXIDANT; PROANTHOCYANIDIN;

D O I：

10.3389/fpls.2022.911203

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Actinidia arguta Sieb.Zucc. is a fruit that is rich in flavonoids. Nevertheless, details of flavonoid formation and the potential mechanism behind flavonoid biosynthesis have not previously been reported. In order to explore the biosynthetic regulation mechanism of flavonoids in A. arguta Sieb.Zucc., we conducted a combination of extensive targeted metabolite analysis and analyzed transcriptomes to determine the flavonoids present and the genes bound up with flavonoid biosynthesis in the two main cultivated varieties of A. arguta Sieb.Zucc. in Northern China. The maturity period is from August to September. A total of 118 flavonoids were found in fruits. Among them, 39 flavonoids were accumulated at significant levels after fruit ripening. Transcriptome analysis indicated that most flavonoid biosynthesis structural genes and certain regulatory genes exhibited differential expression between the two varieties. Correlation analysis of transcriptome and metabolite profiles showed that the ways of expression of 21 differentially expressed genes related to structure and regulation between the 2 varieties were more highly correlated with 7 flavonoids after fruit ripening. These results contribute to the development of A. arguta Sieb.Zucc. as a food and drug homologous functional food.

引用

页数：15

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