DEVELOPMENT OF MICROSATELLITE LOCI FOR BLASTOPHAGA JAVA']JAVANA (AGAONIDAE), THE POLLINATING WASP OF FICUS HIRTA (MORACEAE)

被引：11

作者：

Tian, En-Wei ^{[1
]}

Yu, Hui ^{[1
]}

Zhang, Da-Yong ^{[2
,3
]}

Nason, John D. ^{[4
]}

机构：

[1] Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China

[2] Beijing Normal Univ, State Key Lab Earth Surface Processes & Resource, Beijing 100875, Peoples R China

[3] Beijing Normal Univ, MOE Key Lab Biodivers Sci & Ecol Engn, Beijing 100875, Peoples R China

[4] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA

来源：

AMERICAN JOURNAL OF BOTANY | 2011年 / 98卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Blastophaga; fig wasp; microsatellite; pollinator; primers;

D O I：

10.3732/ajb.1000432

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: To investigate genetic structure of the pollinator and, indirectly, gene flow in its plant host, Blastophaga javana microsatellite primers were developed. Methods and Results: Ten polymorphic microsatellite loci were developed using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol. Numbers of alleles per locus ranged from 2 to 19, with observed and expected heterozygosities ranging from 0.000 to 0.800 and from 0.000 to 0.925, respectively. Conclusions: These markers may be useful for further investigation of population genetics of Blastophaga javana and other congeneric species.

引用

页码：E41 / E43

页数：3

共 7 条

[1] GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research [J].

Peakall, R ;

Smouse, PE .

MOLECULAR ECOLOGY NOTES, 2006, 6 (01) :288-295

[2] GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].

RAYMOND, M ;

ROUSSET, F .

JOURNAL OF HEREDITY, 1995, 86 (03) :248-249

[3]

Rozen S, 2000, Methods Mol Biol, V132, P365

[4] Development and characterization of microsatellite markers for a monoecious Ficus species, Ficus insipida, and cross-species amplification among different sections of Ficus [J].

Vignes, H. ;

Hossaert-Mckey, M. ;

Beaune, D. ;

Fevre, D. ;

Anstett, M. -C. ;

Borges, R. M. ;

Kjellberg, F. ;

Chevallier, M. H. .

MOLECULAR ECOLOGY NOTES, 2006, 6 (03) :792-795

[5] Pollination and parasitism in functionally dioecious figs [J].

Weiblen, GD ;

Yu, DW ;

West, SA .

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2001, 268 (1467) :651-659

[6] Slatkin's Paradox: when direct observation and realized gene flow disagree. A case study in Ficus [J].

Yu, Hui ;

Nason, John D. ;

Ge, Xuejun ;

Zeng, Jiqing .

MOLECULAR ECOLOGY, 2010, 19 (20) :4441-4453

[7] Strategies for microsatellite isolation: a review [J].

Zane, L ;

Bargelloni, L ;

Patarnello, T .

MOLECULAR ECOLOGY, 2002, 11 (01) :1-16

← 1 →