High-speed separation of subnanomolar concentrations of noradrenaline and dopamine using capillary zone electrophoresis with laser-induced fluorescence detection

被引：28

作者：

Bert, L

Robert, F

Denoroy, L

Renaud, B

机构：

[1] CNRS,UMR 105,NEUROPHARMACOL LAB,LYON,FRANCE

[2] UNIV LYON 1,F-69365 LYON,FRANCE

[3] CNRS,SERV CENT ANAL,F-69390 VERNAISON,FRANCE

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 03期

关键词：

capillary zone electrophoresis; dopamine; laser-induced fluorescence; naphthalene-2,3-dicarboxaidehyde; noradrenaline;

D O I：

10.1002/elps.1150170318

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary zone electrophoresis with laser-induced fluorescence detection has been shown to give rapid separations with high resolution and sensitivity. These advantages are documented for catecholamines analysis in the present work, which shows that separation of subnanomolar concentrations of dopamine and noradrenaline (detection limits of 8.6 x 10(-11) M, corresponding to a detected amount of 143 zeptomoles of each catecholamine) can be performed in 82 s with an efficiency of several million theoretical plates.

引用

收藏

页码：523 / 525

页数：3

相关论文

共 21 条

[1]

ABERCROMBIE ED, 1991, MICRODIALYSIS NEUROS, P253

[2] SENSITIVITY ENHANCEMENT FOR CAPILLARY ELECTROPHORESIS [J].

ALBIN, M ;

GROSSMAN, PD ;

MORING, SE .

ANALYTICAL CHEMISTRY, 1993, 65 (10) :A489-A497

[3] DETERMINATION OF CATECHOLAMINES IN SINGLE ADRENAL-MEDULLARY CELLS BY CAPILLARY ELECTROPHORESIS AND LASER-INDUCED NATIVE FLUORESCENCE [J].

CHANG, HT ;

YEUNG, ES .

ANALYTICAL CHEMISTRY, 1995, 67 (06) :1079-1083

[4] ANALYSIS OF GLUTAMATE IN STRIATAL MICRODIALYSATES USING CAPILLARY ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE DETECTION [J].

DAWSON, LA ;

STOW, JM ;

DOURISH, CT ;

ROUTLEDGE, C .

JOURNAL OF CHROMATOGRAPHY A, 1995, 700 (1-2) :81-87

[5] GLASS CHIPS FOR HIGH-SPEED CAPILLARY ELECTROPHORESIS SEPARATIONS WITH SUBMICROMETER PLATE HEIGHTS [J].

EFFENHAUSER, CS ;

MANZ, A ;

WIDMER, HM .

ANALYTICAL CHEMISTRY, 1993, 65 (19) :2637-2642

[6] ANALYSIS OF SINGLE CELLS BY CAPILLARY ELECTROPHORESIS WITH ON COLUMN DERIVATIZATION AND LASER-INDUCED FLUORESCENCE DETECTION [J].

GILMAN, SD ;

EWING, AG .

ANALYTICAL CHEMISTRY, 1995, 67 (01) :58-64

[7]

GROSSMAN PD, 1992, CAPILLARY ELECTROPHO, P3

[8] LASER-INDUCED FLUORESCENCE AND FLUORESCENCE MICROSCOPY FOR CAPILLARY ELECTROPHORESIS ZONE DETECTION [J].

HERNANDEZ, L ;

ESCALONA, J ;

JOSHI, N ;

GUZMAN, N .

JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :183-196

[9] COLLINEAR LASER-INDUCED FLUORESCENCE DETECTOR FOR CAPILLARY ELECTROPHORESIS - ANALYSIS OF GLUTAMIC-ACID IN BRAIN DIALYSATES [J].

HERNANDEZ, L ;

JOSHI, N ;

MURZI, E ;

VERDEGUER, P ;

MIFSUD, JC .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :399-405

[10] IN-VIVO MONITORING OF GLUTAMATE IN THE BRAIN BY MICRODIALYSIS AND CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION [J].

HERNANDEZ, L ;

TUCCI, S ;

GUZMAN, N ;

PAEZ, X .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :393-398