Evaluation of bacterial strains isolated from oil-contaminated soil for production of polyhydroxyalkanoic acids (PHA)

被引：30

作者：

Dalal, Jyotsana ^{[2
]}

Sarma, Priyangshu M. ^{[1
]}

Lavania, Meeta ^{[1
]}

Mandal, Ajoy K. ^{[1
]}

Lal, Banwari ^{[1
]}

机构：

[1] TERI, Microbial Biotechnol Div, New Delhi 110003, India

[2] TERI Univ, Ctr Bioresources & Biotechnol, New Delhi 110070, India

来源：

PEDOBIOLOGIA | 2010年 / 54卷 / 01期

关键词：

Polyhydroxyalkanoic acids; Bacteria; Oil-contaminated soil; LENGTH 3-HYDROXYALKANOIC ACIDS; PSEUDOMONAS-AERUGINOSA; PETROLEUM-HYDROCARBONS; BIOSYNTHESIS; POLYESTERS; BIODEGRADATION; DIVERSITY; SYNTHASES; PLASTICS; SITES;

D O I：

10.1016/j.pedobi.2010.08.004

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Samples of eight geographically distinct soils contaminated with crude oil were screened for polyhydroxyalkanoic acid (PHA) producing bacterial strains. Twenty three bacterial strains were able to accumulate PHA when sodium gluconate or sodium octanoate was used as the sole carbon source. Biochemical tests and 16S rRNA sequencing identified bacteria of the genera Pseudomonas, Acinetobacter, Sphingobacterium, Brochothrix, Caulobacter, Ralstonia, Burkholderia and Yokenella. Three of the bacterial strains have never been reported to produce PHA. The phylogenetic analysis of the PHA synthase (phaC) gene of these bacteria showed a close homology with the phaC gene of different Pseudomonas species. This study indicates that stressed environments like oil-contaminated sites can be potential sources of medium-chain-length PHA producers. (C) 2010 Elsevier GmbH. All rights reserved.

引用

页码：25 / 30

页数：6

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