Combination of tamoxifen and antisense human telomerase RNA inhibits glioma cell proliferation and anti-apoptosis via suppression of telomerase activity

被引：9
|
作者：
Wang, Yingyi [2 ]
Zhou, Zhimin [1 ]
Luo, Hui [2 ]
Wu, Zhifeng [1 ]
Geng, Jiong [1 ]
Miu, Weifeng [1 ]
Pu, Yi [1 ]
Liu, Ning [2 ]
You, Yongping [2 ]
Yang, Zhengxiang [1 ]
机构：
[1] Nanjing Med Univ, Affiliated Wuxi Peoples Hosp, Dept Neurosurg, Wuxi 214023, Peoples R China
[2] Nanjing Med Univ, Affiliated Hosp 1, Dept Neurosurg, Nanjing 210029, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2010年 / 3卷 / 06期
关键词：
glioma; telomerase; telomerase RNA; tamoxifen; combination therapy; BREAST-CANCER; IN-VITRO; GROWTH; HTERT; GLIOBLASTOMA; EXPRESSION; CASPASE-3; THERAPY; SUBUNIT; DEATH;
D O I：
10.3892/mmr.2010.356
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Accumulating evidence indicates that telomerase activity and human telomerase RNA (hTR) play a potentially crucial role in maintaining the malignant progression of human gliomas. Tamoxifen (TAM) induces cell growth inhibition by modulating several cellular activities, including signaling pathways and the cell cycle. In this study, we aimed to evaluate the effect of combination therapy with TAM and antisense hTR on malignant glioma growth in vitro. TAM treatment and the down-regulation of hTR expression by antisense hTR resulted in a significant suppression of cell growth and the induction of cell apoptosis through the inhibition of telomerase activity. The antitumor effect of treatment with TAM alone was found to be mediated in part by the down-regulation of telomerase activity and of PKC and IGF-1 expression. Our results indicate that TAM and antisense hTR may serve as a novel strategy for glioma therapy.
引用
收藏
页码：935 / 940
页数：6
相关论文
共 50 条
  • [21] A novel antisense oligonucleotide anchored on the intronic splicing enhancer of hTERT pre-mRNA inhibits telomerase activity and induces apoptosis in glioma cells
    Fei Wang
    Yajing Cheng
    Chi Zhang
    Guangming Chang
    Xin Geng
    Journal of Neuro-Oncology, 2019, 143 : 57 - 68
  • [22] Apoptosis and Reduced Cell Proliferation of HL-60 Cell Line Caused by Human Telomerase Reverse Transcriptase Inhibition by siRNA
    Miri-Moghaddam, Ebrahim
    Deezagi, Abdolkhaleg
    Soheili, Zahra Sohaila
    Shariati, Parvin
    ACTA HAEMATOLOGICA, 2010, 124 (02) : 72 - 78
  • [23] Designed modulation of sex steroid signaling inhibits telomerase activity and proliferation of human prostate cancer cells
    Verma, Vikas
    Sharma, Vikas
    Singh, Vishal
    Sharma, Siddharth
    Bishnoi, Ajay Kumar
    Chandra, Vishal
    Maikhuri, J. P.
    Dwivedi, Anila
    Kumar, Atul
    Gupta, Gopal
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2014, 280 (02) : 323 - 334
  • [24] Human hepatoma cell telomerase activity inhibition and cell cycle modulation by its RNA component antisense oligodeoxyribonucleotides
    Zhang, RG
    Wang, XW
    Yuan, JH
    Xie, H
    ACTA PHARMACOLOGICA SINICA, 2000, 21 (08) : 742 - 746
  • [25] Curcumin inhibits telomerase activity in human cancer cell lines
    Cui, Shu-Xiang
    Qu, Xian-Jun
    Xie, Yan-Ying
    Zhou, Ling
    Nakata, Munehiro
    Makuuchi, Masatoshi
    Tang, Wei
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2006, 18 (02) : 227 - 231
  • [26] Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line
    Xiao-Hua Fu
    World Journal of Gastroenterology, 2005, (06) : 785 - 790
  • [27] RNA interference targeting NRP-1 inhibits human glioma cell proliferation and enhances cell apoptosis
    Li, Xiangyun
    Tang, Ting
    Lu, Xiaozhe
    Zhou, Houguang
    Huang, Yanyan
    MOLECULAR MEDICINE REPORTS, 2011, 4 (06) : 1261 - 1266
  • [28] Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line
    Fu, Xiao-Hua
    Zhang, Jian-Song
    Zhang, Na
    Zhang, Yang-De
    WORLD JOURNAL OF GASTROENTEROLOGY, 2005, 11 (06) : 785 - 790
  • [29] Downregulation of telomerase activity in human promyelocytic cell line using RNA interference
    E. Miri-Moghaddam
    A. Deezagi
    Z. S. Soheili
    Annals of Hematology, 2009, 88 : 1169 - 1176
  • [30] Curcumin inhibits telomerase activity through human telomerase reverse transcritpase in MCF-7 breast cancer cell line
    Ramachandran, C
    Fonseca, HB
    Jhabvala, P
    Escalon, EA
    Melnick, SJ
    CANCER LETTERS, 2002, 184 (01) : 1 - 6
12345