Optogenetic control of heart muscle in vitro and in vivo

被引：334

作者：

Bruegmann, Tobias ^{[1
]}

Malan, Daniela ^{[1
]}

Hesse, Michael ^{[1
]}

Beiert, Thomas ^{[1
]}

Fuegemann, Christopher J. ^{[1
]}

Fleischmann, Bernd K. ^{[1
]}

Sasse, Philipp ^{[1
]}

机构：

[1] Univ Bonn, Inst Physiol 1, Life & Brain Ctr, D-53115 Bonn, Germany

来源：

NATURE METHODS | 2010年 / 7卷 / 11期

关键词：

EMBRYONIC STEM-CELLS; CARDIOMYOCYTES; STIMULATION; CHANNELRHODOPSIN-2; ENGRAFTMENT; MECHANISM; RESPONSES; MYOCYTES; CHANNEL; TISSUE;

D O I：

10.1038/nmeth.1512

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca(2+) homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.

引用

页码：897 / U45

页数：7

共 19 条

[1] Calcium cycling and signaling in cardiac myocytes [J].

Bers, Donald M. .

ANNUAL REVIEW OF PHYSIOLOGY, 2008, 70 :23-49

[2] Millisecond-timescale, genetically targeted optical control of neural activity [J].

Boyden, ES ;

Zhang, F ;

Bamberg, E ;

Nagel, G ;

Deisseroth, K .

NATURE NEUROSCIENCE, 2005, 8 (09) :1263-1268

[3] Developmental and adult phenotyping directly from mutant embryonic stem cells [J].

George, Sophia H. L. ;

Gertsenstein, Marina ;

Vintersten, Kristina ;

Korets-Smith, Ella ;

Murphy, John ;

Stevens, Mary E. ;

Haigh, Jody J. ;

Nagy, Andras .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (11) :4455-4460

[4] Spatial changes in transmembrane potential during extracellular electrical shocks in cultured monolayers of neonatal rat ventricular myocytes [J].

Gillis, AM ;

Fast, VG ;

Rohr, S ;

Kleber, AG .

CIRCULATION RESEARCH, 1996, 79 (04) :676-690

[5] Targeting and readout strategies for fast optical neural control in vitro and in vivo [J].

Gradinaru, Viviana ;

Thompson, Kimberly R. ;

Zhang, Feng ;

Mogri, Murtaza ;

Kay, Kenneth ;

Schneider, M. Bret ;

Deisseroth, Karl .

JOURNAL OF NEUROSCIENCE, 2007, 27 (52) :14231-14238

[6] Roles of electric field and fiber structure in cardiac electric stimulation [J].

Knisley, SB ;

Trayanova, N ;

Aguel, F .

BIOPHYSICAL JOURNAL, 1999, 77 (03) :1404-1417

[7] Engraftment of engineered ES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium [J].

Kolossov, Eugen ;

Bostani, Toktam ;

Roell, Wilhelm ;

Breitbach, Martin ;

Pillekamp, Frank ;

Nygren, Jens M. ;

Sasse, Philipp ;

Rubenchik, Olga ;

Fries, Jochen W. U. ;

Wenzel, Daniela ;

Geisen, Caroline ;

Xia, Ying ;

Lu, Zhongju ;

Duan, Yaqi ;

Kettenhofen, Ralf ;

Jovinge, Stefan ;

Bloch, Wilhelm ;

Bohlen, Heribert ;

Welz, Armin ;

Hescheler, Juergen ;

Jacobsen, Sten Eirik ;

Fleischmann, Bernd K. .

JOURNAL OF EXPERIMENTAL MEDICINE, 2006, 203 (10) :2315-2327

[8] CARDIOMYOCYTES DIFFERENTIATED IN-VITRO FROM EMBRYONIC STEM-CELLS DEVELOPMENTALLY EXPRESS CARDIAC-SPECIFIC GENES AND IONIC CURRENTS [J].

MALTSEV, VA ;

WOBUS, AM ;

ROHWEDEL, J ;

BADER, M ;

HESCHELER, J .

CIRCULATION RESEARCH, 1994, 75 (02) :233-244

[9] Electrical stimulation of excitable tissue: design of efficacious and safe protocols [J].

Merrill, DR ;

Bikson, M ;

Jefferys, JGR .

JOURNAL OF NEUROSCIENCE METHODS, 2005, 141 (02) :171-198

[10] Channelrhodopsin-2, a directly light-gated cation-selective membrane channel [J].

Nagel, G ;

Szellas, T ;

Huhn, W ;

Kateriya, S ;

Adeishvili, N ;

Berthold, P ;

Ollig, D ;

Hegemann, P ;

Bamberg, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) :13940-13945

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