Cationic lipid-DNA complexes in gene delivery:: from biophysics to biological applications

被引:297
作者
de Lima, MCP [1 ]
Simoes, S
Pires, P
Faneca, H
Düzgünes, N
机构
[1] Univ Coimbra, Dept Biochem, P-3000 Coimbra, Portugal
[2] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3000 Coimbra, Portugal
[3] Univ Coimbra, Pharmaceut Technol Lab, Fac Pharm, P-3000 Coimbra, Portugal
[4] Univ Pacific, Sch Dent, Dept Microbiol, San Francisco, CA 94115 USA
关键词
gene therapy; non-viral vectors; lipoplexes; biological barriers;
D O I
10.1016/S0169-409X(01)00110-7
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Great expectations from the application of gene therapy approaches to human disease have been impaired by the unsatisfactory clinical progress observed. Among others, the use of an efficient carrier for nucleic acid-based medicines is considered to be a determinant factor for the successful application of this promising therapeutic strategy. The drawbacks associated with the use of viral vectors, namely those related with safety problems, have prompted investigators to develop alternative methods for gene delivery, cationic lipid-based systems being the most representative. This review focuses on the various parameters that are considered to be crucial to optimize the use of cationic lipid-DNA complexes for gene therapy purposes. Particular emphasis is devoted to the analysis of the different stages involved in the transfection process, from the biophysical aspects underlying the formation of the complexes to the different biological barriers that need to be surpassed for gene expression to occur. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:277 / 294
页数:18
相关论文
共 91 条
[1]   Nuclear localization signal peptides enhance cationic liposome-mediated gene therapy [J].
Aronsohn, AI ;
Hughes, JA .
JOURNAL OF DRUG TARGETING, 1998, 5 (03) :163-169
[2]   Biological barriers to cellular delivery of lipid-based DNA carriers [J].
Bally, MB ;
Harvie, P ;
Wong, FMP ;
Kong, S ;
Wasan, EK ;
Reimer, DL .
ADVANCED DRUG DELIVERY REVIEWS, 1999, 38 (03) :291-315
[3]   Evidence for three-dimensional interlayer correlations in cationic lipid-DNA complexes as observed by cryo-electron microscopy [J].
Battersby, BJ ;
Grimm, R ;
Huebner, S ;
Cevc, G .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1372 (02) :379-383
[5]   EFFICIENT GENE-TRANSFER INTO MAMMALIAN PRIMARY ENDOCRINE-CELLS WITH LIPOPOLYAMINE-COATED DNA [J].
BEHR, JP ;
DEMENEIX, B ;
LOEFFLER, JP ;
MUTUL, JP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (18) :6982-6986
[6]   A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA [J].
Brandén, LJ ;
Mohamed, AJ ;
Smith, CIE .
NATURE BIOTECHNOLOGY, 1999, 17 (08) :784-787
[7]   Enhancement of polylysine-mediated transferrinfection by nuclear localization sequences: Polylysine does not function as a nuclear localization sequence [J].
Chan, CK ;
Jans, DA .
HUMAN GENE THERAPY, 1999, 10 (10) :1695-1702
[8]   Receptor ligand-facilitated gene transfer: Enhancement of liposome-mediated gene transfer and expression by transferrin [J].
Cheng, PW .
HUMAN GENE THERAPY, 1996, 7 (03) :275-282
[9]   Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum [J].
Crook, K ;
Stevenson, BJ ;
Dubouchet, M ;
Porteous, DJ .
GENE THERAPY, 1998, 5 (01) :137-143
[10]   Signalling and transport through the nuclear membrane [J].
Csermely, P ;
Schnaider, T ;
Szanto, I .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES, 1995, 1241 (03) :425-451