Development of Loop-Mediated Isothermal Amplification-Based Lateral Flow Device Method for the Detection of Malaria

被引：15

作者：

Mallepaddi, Prudhvi Chand ^{[1
,2
]}

Lai, Meng-Yee ^{[3
]}

Podha, Sudhakar ^{[2
]}

Ooi, Choo-Huck ^{[4
]}

Liew, Jonathan Wee-Kent ^{[3
]}

Polavarapu, Rathnagiri ^{[1
,2
]}

Lau, Yee-Ling ^{[3
]}

机构：

[1] Genomix Mol Diagnost Pvt Ltd, Hyderabad, Telangana, India

[2] Acharya Nagarjuna Univ, Dept Biotechnol, Guntur, India

[3] Univ Malaya, Dept Parasitol, Fac Med, Kuala Lumpur 50603, Malaysia

[4] Sarawak State Hlth Dept, Jalan Diplomatik, Kuching, Malaysia

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2018年 / 99卷 / 03期

关键词：

PLASMODIUM-FALCIPARUM; ASSAY; IDENTIFICATION; DIAGNOSIS;

D O I：

10.4269/ajtmh.18-0177

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amidite-labeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes to complete, LAMP assay exhibited high sensitivity, as the detection limit was 0.01 pg/mu L for all five Plasmodium species. It was demonstrated that all Plasmodium knowlesi (N = 90) and Plasmodium vivax (N = 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples (N = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD represents a robust approach with potential suitability for use in resource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future.

引用

页码：704 / 708

页数：5

共 18 条

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