One-Pot Polymerase Chain Reaction with Gold Nanoparticles for Rapid and Ultrasensitive DNA Detection

被引：28

作者：

Cai, Miao ^{[1
]}

Li, Feng ^{[1
]}

Zhang, Yan ^{[1
]}

Wang, Qiangbin ^{[1
]}

机构：

[1] Chinese Acad Sci, i Lab, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China

来源：

NANO RESEARCH | 2010年 / 3卷 / 08期

关键词：

deoxyribonucleic acid ( DNA) detection; polymerase chain reaction (PCR); Au nanoparticle; colorimetric; ELECTRICAL DETECTION; OLIGONUCLEOTIDES; SURFACE; ARRAYS; PCR;

D O I：

10.1007/s12274-010-0016-6

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid ( DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

引用

页码：557 / 563

页数：7

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