Artificial microRNA-mediated resistance to cucumber green mottle mosaic virus in Nicotiana benthamiana

被引：17
作者：
Liang, Chaoqiong [1 ,2 ]
Hao, Jianjun [3 ]
Li, Jianqiang [1 ]
Baker, Barbara [4 ,5 ]
Luo, Laixin [1 ]
机构：
[1] China Agr Univ, Coll Plant Protect, Beijing Key Lab Seed Dis Testing & Control, Beijing 100193, Peoples R China
[2] China Agr Univ, Coll Hort, Beijing 100193, Peoples R China
[3] Univ Maine, Sch Food & Agr, Orono, ME 04469 USA
[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[5] ARS, USDA, Plant Gene Express Ctr, Albany, CA 94710 USA
来源：
PLANTA | 2019年 / 250卷 / 05期
基金：
美国国家科学基金会;
关键词：
Cucumber green mottle mosaic virus; Artificial microRNA; RNA silencing; Virus resistance; Crop protection; HIGHLY EFFICIENT; TARGET; PLANTS; RNA; EXPRESSION; SEQUENCE; WHEAT; ARABIDOPSIS; BIOGENESIS; SCREENS;
D O I：
10.1007/s00425-019-03252-w
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Main conclusionWe describe a Nicotiana benthamiana system for rapid identification of artificial microRNA (amiRNA) to control cucumber green mottle mosaic virus (CGMMV) disease.AbstractAlthough artificial miRNA technology has been used to control other viral diseases, it has not been applied to reduce severe cucumber green mottle mosaic virus (CGMMV) disease and crop loss in the economically important cucurbits. We used our system to identify three amiRNAs targeting CGMMV RNA (amiR1-CP, amiR4-MP and amiR6-Rep) and show that their expression reduces CGMMV replication and disease in virus-infected plants. This work streamlines the process of generating amiRNA virus-resistant crops and can be broadly applied to identify active antiviral amiRNAs against a broad spectrum of viruses to control disease in diverse crops.
引用
收藏
页码：1591 / 1601
页数：11
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