Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris

被引：3

作者：

Cheng, Junhao ^{[1
]}

Wu, Mingkun ^{[1
]}

Zhong, Ren ^{[1
]}

Si, Dayong ^{[1
]}

Meng, Geng ^{[1
]}

Zhang, Rijun ^{[1
]}

Zhang, Yueping ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Coll Anim Sci & Technol, Lab Feed Biotechnol,State Key Lab Anim Nutr, Beijing 100193, Peoples R China

来源：

BIOTECHNIQUES | 2021年 / 71卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Escherichia coli; Golden Gate; heterologous expression; Pichia pastoris; quick exon assembly;

D O I：

10.2144/btn-2021-0039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficient and cost-effective assembly of multiple exons of interest genes into different expression plasmids in both Escherichia coli and Pichia pastoris. The high cloning efficiency and low cost of the system make it ideal for a novel workflow for the assembly of intron-bearing genes for expression in two different expression hosts.

引用

页数：6

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