The oncogenic role of microRNA-500a in colorectal cancer

被引：7
作者：
Li, Yang [1 ]
Chen, Zhong [2 ]
机构：
[1] Chengdu Shangjin Nan Fu Hosp, Dept Gen Surg 1, Chengdu 510117, Sichuan, Peoples R China
[2] Sichuan Univ, West China Hosp, Dept Gastrointestinal Surg, 37 Guoxue Alley, Chengdu 610041, Sichuan, Peoples R China
来源：
ONCOLOGY LETTERS | 2020年 / 19卷 / 03期
关键词：
microRNA-500a; oncogenic role; cellular proliferation; colorectal cancer; MTT COLORIMETRIC ASSAY; PTEN; PROGNOSIS; INVASION; PATHWAY; PCR;
D O I：
10.3892/ol.2020.11291
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Colorectal cancer (CRC) is a common and lethal disease, and microRNAs (miRNAs/miRs) serve an important role in the pathogenesis of CRC. miR-500a is a novel miRNA, and although its function has been studied in hepatocellular carcinoma, the function of miR-500a in CRC remains unknown. In the present study, the function of miR-500a in CRC was investigated. The expression levels of miR-500a in cells and tissues were investigated using reverse transcription-quantitative PCR. Cell proliferation was tested using MTT assay and migration was assessed using Transwell systems. The results revealed that there were higher levels of miR-500a in tumor tissue compared with in normal tissue. Inhibition of miR-500a suppressed cell growth and migration, whereas overexpression of miR-500a promoted cell growth and migration. Additionally, it was revealed that miR-500a may target the 3 '-untranslated region of the phosphatase and tensin homolog gene. In conclusion, the present study demonstrated that miR-500a may serve an oncogenic role in CRC.
引用
收藏
页码：1799 / 1805
页数：7
相关论文
共 35 条
[1]   Role of exogenous and endogenous hormones in endometrial cancer - Review of the evidence and research perspectives [J].
Akhmedkhanov, A ;
Zeleniuch-Jacquotte, A ;
Toniolo, P .
HUMAN FERTILITY AND REPRODUCTION: THE OOCYTE, THE EMBRYO, AND THE UTERUS, 2001, 943 :296-315
[2]   MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer [J].
Asangani, I. A. ;
Rasheed, S. A. K. ;
Nikolova, D. A. ;
Leupold, J. H. ;
Colburn, N. H. ;
Post, S. ;
Allgayer, H. .
ONCOGENE, 2008, 27 (15) :2128-2136
[3]   MicroRNA-500a Promotes the Progression of Hepatocellular Carcinoma by Post-Transcriptionally Targeting BID [J].
Bao, Leilei ;
Zhang, Mingjian ;
Han, Shu ;
Zhan, Yangyang ;
Guo, Wenyuan ;
Teng, Fei ;
Liu, Fang ;
Guo, Meng ;
Zhang, Luding ;
Ding, Guoshan ;
Wang, Quanxing .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 47 (05) :2046-2055
[4]   MicroRNAs: Target Recognition and Regulatory Functions [J].
Bartel, David P. .
CELL, 2009, 136 (02) :215-233
[5]   The PTEN/PI3K/AKT signalling pathway in cancer, therapeutic implications [J].
Carnero, Amancio ;
Blanco-Aparicio, Carmen ;
Renner, Oliver ;
Link, Wolfgang ;
Leal, Juan F. M. .
CURRENT CANCER DRUG TARGETS, 2008, 8 (03) :187-198
[6]   MicroRNA in Prostate, Bladder, and Kidney Cancer: A Systematic Review [J].
Catto, James W. F. ;
Alcaraz, Antonio ;
Bjartell, Anders S. ;
White, Ralph De Vere ;
Evans, Christopher P. ;
Fussel, Susanne ;
Hamdy, Freddie C. ;
Kallioniemi, Olli ;
Mengual, Lourdes ;
Schlomm, Thorsten ;
Visakorpi, Tapio .
EUROPEAN UROLOGY, 2011, 59 (05) :671-681
[7]   PTEN and the PI3-Kinase Pathway in Cancer [J].
Chalhoub, Nader ;
Baker, Suzanne J. .
ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE, 2009, 4 :127-150
[8]   PTEN nuclear localization is regulated by oxidative stress and mediates p53-dependent tumor suppression [J].
Chang, Chun-Ju ;
Mulholland, David J. ;
Valamehr, Bahram ;
Mosessian, Sherly ;
Sellers, William R. ;
Wu, Hong .
MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (10) :3281-3289
[9]  
Deocampo N D, 2003, Minerva Endocrinol, V28, P145
[10]   miR-500a-5p regulates oxidative stress response genes in breast cancer and predicts cancer survival [J].
Esposti, Davide Degli ;
Aushev, Vasily N. ;
Lee, Eunjee ;
Cros, Marie-Pierre ;
Zhu, Jun ;
Herceg, Zdenko ;
Chen, Jia ;
Hernandez-Vargas, Hector .
SCIENTIFIC REPORTS, 2017, 7
1234