Recombinant human bone morphogenetic protein 2 enhances bone heating in an experimental model of fractures at risk of non-union

被引:33
作者
Eckardt, H [1 ]
Christensen, KS
Lind, M
Hansen, ES
Hall, DWR
Hvid, I
机构
[1] Aalborg Hosp, Dept Orthoped, DK-9000 Aalborg, Denmark
[2] Aarhus Univ Hosp, Dept Orthoped, DK-8000 Aarhus, Denmark
[3] Yamanouchi Europe BV, Deiderdorp, Netherlands
来源
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED | 2005年 / 36卷 / 04期
关键词
bone morphogenetic protein 2 (BMP-2); growth factor; non-union; delayed union; rabbit; tibia; bone blood flow; microspheres; osteoinduction; hyaluronic acid;
D O I
10.1016/j.injury.2004.10.019
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Introduction: Identification of patients at risk of developing non-union and institution of procedures preventing non-union could be attractive in routine fracture management. We investigated whether recombinant human bone morphogenetic protein (rhBMP-2) delivered in a hyaluronic acid carrier could prevent non-union development in an experimental non-union model, which simulates the clinical situation of open mid-tibial fractures. Methods: Sixteen rabbits underwent a standard non-union operation comprising midtibial osteotomy, excision of periosteum and endosteum, and plate fixation. Before closure of the wound eight rabbits received interfragmentary deposition of 200 mu g rhBMP-2 delivered in a hyaluronan get carrier, and eight rabbits received get carrier alone. Results: After 7 weeks, torsional failure moment of the osteotomy and energy absorbed at failure, macroscopic and radiographic appearance, callus area, and interfragmentary bone volume fraction confirmed that rhBMP-2 delivery significantly improved bone heating. Blood flow at the osteotomy site, measured using radiolabelled microspheres, was not higher in the united osteotomies than in non-united osteotomies. Discussion: rhBMP-2 delivered in a hyaluronic acid carrier-induced formation of competent bone in an experimental model of compromised heating. We, therefore, propose interfragmentary deposition of rhBMP-2 delivered in a hyaluronic acid carrier to patients encountering fractures at risk of non-union or delayed union. (c) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:489 / 494
页数:6
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