Production Process for Stem Cell Based Therapeutic Implants: Expansion of the Production Cell Line and Cultivation of Encapsulated Cells

被引：26

作者：

Weber, C. ^{[2
]}

Pohl, S. ^{[2
]}

Poertner, R. ^{[3
]}

Pino-Grace, Pablo ^{[2
]}

Freimark, D. ^{[2
]}

Wallrapp, C. ^{[4
]}

Geigle, P. ^{[4
]}

Czermak, P. ^{[1
,2
]}

机构：

[1] Kansas State Univ, Dept Chem Engn, Manhattan, KS 66506 USA

[2] Univ Appl Sci Giessen Friedberg, Inst Biopharmaceut Technol, Giessen, Germany

[3] Tech Univ Hamburg, Inst Bioproc & Biosyst Technol, Hamburg, Germany

[4] CellMed AG, Alzenau, Germany

来源：

BIOREACTOR SYSTEMS FOR TISSUE ENGINEERING II: STRATEGIES FOR THE EXPANISON AND DIRECTED DIFFERENTIATION OF STEM CELLS | 2010年 / 123卷

关键词：

Cell therapy; Mesenchymal stem cells; Encapsulation; Fixed bed bioreactor; Glass carrier; DIFFERENTIATION; CULTURES; GROWTH; DESIGN;

D O I：

10.1007/10_2009_25

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell based therapy promises the treatment of many diseases like diabetes mellitus, Parkinson disease or stroke. Microencapsulation of the cells protects them against host-vs-graft reactions and thus enables the usage of allogenic cell lines for the manufacturing of cell therapeutic implants. The production process of such implants consists mainly of the three steps expansion of the cells, encapsulation of the cells, and cultivation of the encapsulated cells in order to increase their vitality and thus quality. This chapter deals with the development of fixed-bed bioreactor-based cultivation procedures used in the first and third step of production. The bioreactor system for the expansion of the stem cell line (hMSC-TERT) is based on non-porous glass spheres, which support cell growth and harvesting with high yield and vitality. The cultivation process for the spherical cell based implants leads to an increase of vitality and additionally enables the application of a medium-based differentiation protocol.

引用

页码：143 / 162

页数：20

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