Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions: A role for T-cadherin in tumor angiogenesis

被引：55

作者：

Ghosh, Sourabh

Josh, Manjunath B.

Ivanov, Danila

Feder-Mengus, Chantal

Spagnoli, Giulio C.

Martin, Ivan

Erne, Paul

Resink, Therese J. ^{[1
]}

机构：

[1] Univ Basel Hosp, Dept Res, Lab Signal Trasduct, CH-4031 Basel, Switzerland

[2] Univ Basel Hosp, Dept Surg & Res, ICFS, CH-4031 Basel, Switzerland

[3] Kantonsspital, Div Cardiol, Luzern, Switzerland

来源：

FEBS LETTERS | 2007年 / 581卷 / 23期

关键词：

melanoma cell; tumor spheroid; angiogenesis; T-cadherin;

D O I：

10.1016/j.febslet.2007.08.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study addresses establishment of an "in vitro" melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRTPCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A] and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor micro-environment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis. (C) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：4523 / 4528

页数：6

共 35 条

[1] An adiponectin receptor, T-cadherin, was selectively expressed in intratumoral capillary endothelial cells in hepatocellular carcinoma: possible cross talk between T-cadherin and FGF-2 pathways [J].