Interactions of two cytotoxic organoruthenium(II) complexes with G-quadruplex

被引：5

作者：

Sersen, Sara ^{[1
]}

Sket, Primoz ^{[2
,3
]}

Plavec, Janez ^{[1
,2
,3
]}

Turel, Iztok ^{[1
,2
]}

机构：

[1] Univ Ljubljana, Fac Chem & Chem Technol, Vecna Pot 113, SI-1000 Ljubljana, Slovenia

[2] EN FIST Ctr Excellence, SI-1000 Ljubljana, Slovenia

[3] Natl Inst Chem, Slovenian NMR Ctr, Hajdrihova 19, SI-1000 Ljubljana, Slovenia

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2016年 / 160卷

关键词：

Organoruthenium complex; Anticancer activity; UV melting; G-quadruplex; NMR study; RUTHENIUM(II) ARENE COMPLEXES; CRYSTAL-STRUCTURE; ANTICANCER COMPLEXES; DNA; BINDING; TELOMERASE; LIGANDS; OLIGONUCLEOTIDES; INTERCALATION; EQUILIBRIUM;

D O I：

10.1016/j.jinorgbio.2015.11.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two previously isolated cytotoxic complexes [(eta(6)-p-cymene)Ru(kappa(2)-CF3COCHCOC5H3O)L](n+) (L = Cl (1); n = 0, pta (2) (pta 1,3,5-triaza-7-phosphaadamantane); n = 1) were investigated for their selectivity and ability to interact with DNA G-quadruplex adopted by d[G(3)ATG(3)ACACAG(4)ACG(3)] (3) whose topology exhibits diagonal, edge-type and double-chain reversal loops. Structural changes were followed using high-resolution NMR techniques in the presence of 1 and 2. Results showed weak interaction between the organoruthenium complexes and G-quadruplex. Moreover, no significant changes in thermal stability were confirmed by a UV-melting assay for both 1 and 2. These findings emphasize that anticancer activity of Ru(II) complexes may not be correlated with binding to nucleic acid like G-quadruplex. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：70 / 77

页数：8

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