Novel combination proliposomes containing tobramycin and clarithromycin effective against Pseudomonas aeruginosa biofilms

被引：13
作者：
Ye, Tiantian [1 ]
Sun, Siping [2 ]
Sugianto, Tiffanie Daisy [2 ]
Tang, Patricia [2 ]
Parumasivam, Thaigarajan [2 ]
Chang, Yoon Kyung [2 ]
Astudillo, Ariel [2 ]
Wang, Shujun [1 ]
Chan, Hak-Kim [2 ]
机构：
[1] Shenyang Pharmaceut Univ, Sch Pharm, Dept Pharmaceut, 103 Wen Hua Rd, Shenyang 110016, Liaoning, Peoples R China
[2] Univ Sydney, Sch Pharm, Adv Drug Delivery Grp, Sydney, NSW, Australia
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2018年 / 552卷 / 1-2期
关键词：
Combination proliposome; Tobramycin; Clarithromycin; Spray dying; Synergy; Pseudomonas aeruginosa; IN-VITRO; CYSTIC-FIBROSIS; LIPOSOMAL CIPROFLOXACIN; EFFICACY; INFECTIONS; MACROLIDE; CULTURE; MODEL; VIVO;
D O I：
10.1016/j.ijpharm.2018.09.061
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Tobramycin (FOB) and clarithromycin (CLA) can potentially be used synergistically for the treatment of respiratory infections caused by Pseudomonas aeruginosa (P. aeruginosa) in cystic fibrosis (CF) patients. This study aimed to develop a novel combination proliposome formulation (TOB/CLA-CPROLips) containing both hydrophilic TOB and hydrophobic CLA via a core-carrier approach. The combination proliposomes were produced by spray drying a suspension comprising spray-driedmannitol (SD-MAN, 0.45%) and spray-dried tobramycin (SDTOB, 0.05%) particles suspended in an ethanolic lipid solution of CLA (0.05%). The lipid layer coated on the surface of the dry proliposome particles conferred moisture protection and sustained drug release properties in comparison to the pure drugs. The optimized TOB/CLA-CPROLips formulation was stable after 3 months of storage at 60% relative humidity (RH) and 25 degrees C. The combination drug proliposomes showed a synergistic antimicrobial activity against planktonic cells and biofilm cultures of P. aeruginosa. In conclusion, the core-carrier method coupled with spray-drying provided a novel approach for the preparation of combination antibiotics proliposomes.
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收藏
页码：130 / 138
页数：9
相关论文
共 29 条
  • [1] Efficacy and Safety of Liposomal Clarithromycin and Its Effect on Pseudomonas aeruginosa Virulence Factors
    Alhajlan, Mai
    Alhariri, Moayad
    Omri, Abdelwahab
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (06) : 2694 - 2704
  • [2] Incorporation of Farnesol Significantly Increases the Efficacy of Liposomal Ciprofloxacin against Pseudomonas aeruginosa Biofilms in Vitro
    Bandara, H. M. H. N.
    Herpin, M. J.
    Kolacny, D., Jr.
    Harb, A.
    Romanovicz, D.
    Smyth, H. D. C.
    [J]. MOLECULAR PHARMACEUTICS, 2016, 13 (08) : 2760 - 2770
  • [3] Mannitol Enhances Antibiotic Sensitivity of Persister Bacteria in Pseudomonas aeruginosa Biofilms
    Barraud, Nicolas
    Buson, Alberto
    Jarolimek, Wolfgang
    Rice, Scott A.
    [J]. PLOS ONE, 2013, 8 (12):
  • [4] Doring Gerd, 2004, J Cyst Fibros, V3, P67, DOI 10.1016/j.jcf.2004.03.008
  • [5] CLINICAL EVALUATION OF AMINOGLYCOSIDE TOXICITY - TOBRAMYCIN VERSUS GENTAMICIN, A PRELIMINARY-REPORT
    FEE, WE
    VIERRA, V
    LATHROP, GR
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1978, 4 : 31 - 36
  • [6] The potential of liposorne-encapsulated ciprofloxacin as a tularemia therapy
    Hamblin, Karleigh A.
    Wong, Jonathan P.
    Blanchard, James D.
    Atkins, Helen S.
    [J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2014, 4
  • [7] Inhaled medication and inhalation devices for lung disease in patients with cystic fibrosis: A European consensus
    Heijerman, Harry
    Westerman, Elsbeth
    Conway, Steven
    Touw, Daan
    Doring, Gerd
    [J]. JOURNAL OF CYSTIC FIBROSIS, 2009, 8 (05) : 295 - 315
  • [8] Cystic fibrosis and other respiratory diseases of impaired mucus clearance
    Livraghi, Alessandra
    Randell, Scott H.
    [J]. TOXICOLOGIC PATHOLOGY, 2007, 35 (01) : 116 - 129
  • [9] Liposomal tobramycin against pulmonary infections of Pseudomonas aeruginosa:: a pharmacokinetic and efficacy study following single and multiple intratracheal administrations in rats
    Marier, JF
    Brazier, JL
    Lavigne, J
    Ducharme, MP
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 52 (02) : 247 - 252
  • [10] In vitro activities of amphotericin B deoxycholate and liposomal amphotericin B against 604 clinical yeast isolates
    Montagna, Maria Teresa
    Lovero, Grazia
    Coretti, Caterina
    De Giglio, Osvalda
    Martinelli, Domenico
    Bedini, Andrea
    Delia, Mario
    Rosato, Antonio
    Codeluppi, Mauro
    Caggiano, Giuseppina
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2014, 63 : 1638 - 1643
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