ROLES OF POLYSIALIC ACID IN MIGRATION AND DIFFERENTIATION OF NEURAL STEM CELLS

被引：23

作者：

Angata, Kiyohiko ^{[1
]}

Fukuda, Minoru ^{[1
]}

机构：

[1] Sanford Burnham Med Res Inst, Canc Res Ctr, Tumor Microenvironm Program, La Jolla, CA USA

来源：

METHODS IN ENZYMOLOGY, VOL 479: FUNCTIONAL GLYCOMICS | 2010年 / 479卷

关键词：

CENTRAL-NERVOUS-SYSTEM; ADHESION MOLECULE; PSA-NCAM; ADULT NEUROGENESIS; DENTATE GYRUS; RAT HIPPOCAMPUS; OLFACTORY-BULB; PRECURSORS; EXPRESSION; PLASTICITY;

D O I：

10.1016/S0076-6879(10)79002-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysialic acid, a homopolymer of alpha 2,8-linked sialic acid, is one of the carbohydrates expressed on neural precursors in the embryonic and adult brain. Polysialic acid, synthesized by two polysialyltransferases (ST8Siall and ST8SialV), mainly modulates functions of the neural cell adhesion molecule (NCAM). Polysialic acid-deficient mice demonstrated that polysialylated NCAM plays crucial roles in various steps of neural development, such as cell survival and cell migration of neural precursors, neuronal guidance, and synapse formation. However, the mechanisms of the diverse phenotypes and molecules affected by polysialic acid remain to be defined. To study the roles of polysialic acid on neural stem cells, analyses of neural stem cells from polysialic acid-deficient and NCAM-deficient mice are useful. Here, we describe how to prepare neural precursor cells from mouse brain and how to analyze migration and differentiation of neurosphere cells in vitro.

引用

页码：25 / 36

页数：12

共 40 条

[1] The Culture of Neural Stem Cells [J].

Ahmed, Sohail .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2009, 106 (01) :1-6

[2] Polysialyltransferases: major players in polysialic acid synthesis on the neural cell adhesion molecule [J].

Angata, K ;

Fukuda, M .

BIOCHIMIE, 2003, 85 (1-2) :195-206

[3] Sialyltransferase ST8Sia-II assembles a subset of polysialic acid that directs hippocampal axonal targeting and promotes fear behavior [J].

Angata, K ;

Long, JM ;

Bukalo, O ;

Lee, W ;

Dityatev, A ;

Wynshaw-Boris, A ;

Schachner, M ;

Fukuda, M ;

Marth, JD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (31) :32603-32613

[4]

Angata K., 2005, NEUROGLYCOBIOLOY, P1

[5] Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice [J].

Angata, Kiyohiko ;

Lee, Wenjau ;

Mitoma, Junya ;

Marth, Jamey D. ;

Fukuda, Minoru .

FUNCTIONAL GLYCOMICS, 2006, 417 :25-+

[6] Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development [J].

Angata, Kiyohiko ;

Huckaby, Valerie ;

Ranscht, Barbara ;

Terskikh, Alexey ;

Marth, Jamey D. ;

Fukuda, Minoru .

MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (19) :6659-6668

[7] PSA-NCAM in mammalian structural plasticity and neurogenesis [J].

Bonfanti, Luca .

PROGRESS IN NEUROBIOLOGY, 2006, 80 (03) :129-164

[8] NCAM in Neuropsychiatric and Neurodegenerative Disorders [J].

Brennaman, Leann H. ;

Maness, Patricia F. .

STRUCTURE AND FUNCTION OF THE NEURAL CELL ADHESION MOLECULE NCAM, 2010, 663 :299-317

[9] Negative regulation of central nervous system myelination by polysialylated-neural cell adhesion molecule [J].

Charles, P ;

Hernandez, MP ;

Stankoff, B ;

Aigrot, MS ;

Colin, C ;

Rougon, G ;

Zalc, B ;

Lubetzki, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (13) :7585-7590

[10]

Chazal G, 2000, J NEUROSCI, V20, P1446

← 1 2 3 4 →