Occurrence of SARS-CoV-2 RNA in Six Municipal Wastewater Treatment Plants at the Early Stage of COVID-19 Pandemic in The United States

被引:25
作者
Sherchan, Samendra P. [1 ]
Shahin, Shalina [1 ]
Patel, Jeenal [1 ]
Ward, Lauren M. [1 ]
Tandukar, Sarmila [2 ]
Uprety, Sital [3 ]
Schmitz, Bradley W. [4 ]
Ahmed, Warish [5 ]
Simpson, Stuart [6 ]
Gyawali, Pradip [7 ]
机构
[1] Tulane Univ, Dept Environm Hlth Sci, 1440 Canal St,Suite 2100, New Orleans, LA 70112 USA
[2] Policy Res Inst, Kathmandu 44600, Nepal
[3] Eawag, Swiss Fed Inst Aquat Sci & Technol, Uberlandstr 133, CH-8600 Dubendorf, Switzerland
[4] Univ Arizona, Yuma Ctr Excellence Desert Agr YCEDA, 6425 W 8th St, Yuma, AZ 85364 USA
[5] CSIRO Land & Water Ecosci Precinct, 41 Boggo Rd, Dutton Pk, Qld 4102, Australia
[6] CSIRO Land & Water, Lucas Heights, NSW 2234, Australia
[7] Inst Environm Sci & Res Ltd, Porirua 5240, New Zealand
来源
PATHOGENS | 2021年 / 10卷 / 07期
关键词
COVID-19; SARS-CoV-2; RNA; wastewater; transmission risk; wastewater treatment;
D O I
10.3390/pathogens10070798
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A-F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April-July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.
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页数:9
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