Interaction of Co, Mn, Mg and Al with d(GCCCATGGC) and d(CCGGGCCCGG): a spectroscopic study

被引:13
作者
Champion, CS
Kumar, D
Rajan, MT
Rao, KSJ
Viswamitra, MA
机构
[1] Indian Inst Sci, Dept Phys, Bangalore 560012, Karnataka, India
[2] Cent Food Technol Res Inst, Dept Biochem & Nutr, Mysore 570013, Karnataka, India
[3] Indian Inst Sci, Jawaharlal Nehru Ctr Adv Sci Res, Bangalore 560012, Karnataka, India
关键词
DNA; interactions; transitions; spectroscopy; trace elements;
D O I
10.1007/s000180050177
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Spectroscopic study of the interactions of metal ions, Co, Mn, Mg and Al with d(GCCCATGGGC) and d(CCGGGCCCGG) revealed the following. Metal ions Mn, Al and Mg at the lowest concentrations enhanced the t(m) of oligomers, whereas Mn and Mg at higher concentrations decreased the t(m). Co enhanced the t(m) of oligomers at higher concentrations. The studies have also indicated that Mn at lower concentrations displaced EtBr fluorescence, Mg and Co at moderate concentrations and Al only at higher concentrations. Addition of Co, Mn, Mg and Al altered the bands of the circulars dichroism (CD) spectra of the oligomers in a concentration-dependent manner. The CD spectra of d(GCCCATGGGC) and d(CCGGGCCCGG) indicated B and Z forms of DNA, respectively, in contrast to the A form observed in the crystal structures. Mg and Co at different ionic strength induced Z-B transition in d(CCGGGCCCGG), while Al at higher concentrations induced a Z-A transition. Mn did not induce any transition. This is the first report to show that Al causes structural transitions in sequence-specific oligomers and has strong binding ability with CC-rich euchromatin oligomers.
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页码:488 / 496
页数:9
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