How to design cell-based biosensors using the sol-gel process

被引：48

作者：

Depagne, Christophe ^{[1
]}

Roux, Cecile ^{[1
]}

Coradin, Thibaud ^{[1
]}

机构：

[1] Univ Paris 06, CNRS, Coll France, UPMC, F-75005 Paris, France

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2011年 / 400卷 / 04期

关键词：

Encapsulation; Living cells; Sol-gel; Biosensors; Silica; RECOMBINANT ESCHERICHIA-COLI; SILICA MATRICES; LIVING BACTERIA; WHOLE CELLS; ENCAPSULATION; IMMOBILIZATION; PROTEINS; VIABILITY; BIOCERS; BIOENCAPSULATION;

D O I：

10.1007/s00216-010-4351-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Inorganic gels formed using the sol-gel process are promising hosts for the encapsulation of living organisms and the design of cell-based biosensors. However, the possibility to use the biological activity of entrapped cells as a biological signal requires a good understanding and careful control of the chemical and physical conditions in which the organisms are placed before, during, and after gel formation, and their impact on cell viability. Moreover, it is important to examine the possible transduction methods that are compatible with sol-gel encapsulated cells. Through an updated presentation of the current knowledge in this field and based on selected examples, this review shows how it has been possible to convert a chemical technology initially developed for the glass industry into a biotechnological tool, with current limitations and promising specificities.

引用

页码：965 / 976

页数：12

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