Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation

被引：22
作者：
Akhtar, Mahmood H.
Mir, Tanveer A.
Gurudatt, N. G.
Chung, Saeromi
Shim, Yoon-Bo [1 ]
机构：
[1] Pusan Natl Univ, Dept Chem, Busan 46241, South Korea
来源：
BIOSENSORS & BIOELECTRONICS | 2016年 / 85卷
基金：
新加坡国家研究基金会;
关键词：
NADH sensor; Human serum; Tumorigenic cells; EDTA; Polymer-GO; NICOTINAMIDE ADENINE-DINUCLEOTIDE; COMPOSITE-MATERIALS; DISEASE; NAD(+); LAYER;
D O I：
10.1016/j.bios.2016.05.045
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A robust amperometric sensor for beta-nicotinamide adenine dinucleotide (NADH) detection was developed through the organic complex formation with ethylenediaminetetraacetic acid (EDTA) bonded on the polyethylenimine (PEI)/activated graphene oxide (AGO) layer. The EDTA immobilized sensor probe (GCE/AGO/PEI-EDTA) revealed a catalytic property towards NADH oxidation that allows for the highly sensitive electrochemical detection of NADH at a low oxidation potential. Surface characterization demonstrated that the negatively charged AGO acted as nanofillers in the positively charged PEI matrix through the charge interaction. The immobilization of EDTA on the polymer layer provided more surface area for NADH to interact with through the enhanced chemical interlocking between them. We observed the strong interaction between NADH and EDTA on the AGO/PEI layer using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS), and the calculation of the minimized energy for complex formation. The dynamic range of NADH was determined to be between 0.05 mu M and 500 mu M with a detection limit (LD) of 20.0 +/- 1.1 nM. The reliability of the developed sensor for biomedical applications was examined by detecting NADH in tumorigenic lung epithelial cells using the standard addition method. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：488 / 495
页数：8
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