Induction of time- and dose-dependent oxidative stress of triazophos to brain and liver in zebrafish (Danio rerio)

被引:32
作者
Wang, Guihua [1 ]
Xiong, Dongmei [1 ]
Wu, Manni [1 ]
Wang, Lixin [1 ]
Yang, Jing [2 ]
机构
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
[2] China Three Gorges Corp, Chinese Sturgeon Res Inst, Hubei Key Lab Three Gorges Project Conservat Fish, Yichang 443100, Hubei, Peoples R China
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2020年 / 228卷
关键词
Triazophos; Danio rerio; Oxidative stress; Enzyme activity; Gene expression; Lipid peroxidation; ANTIOXIDANT ENZYME-ACTIVITIES; GENE-EXPRESSION; CYPRINUS-CARPIO; ACUTE TOXICITY; COMMON CARP; DNA-DAMAGE; RISK-ASSESSMENT; PESTICIDES; EXPOSURE; EMBRYOS;
D O I
10.1016/j.cbpc.2019.108640
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Extensive use of triazophos for the chemical control of pests in agriculture or aquaculture might strongly disturb the aquatic environment due to residue accumulation through various routes like surface run-off, spray-drift and effluent from factories, which have potential negative effects to non-target aquatic organisms. Previous studies have documented the antioxidative effects of triazophos to mammals, however, the oxidative toxicity of triazophos to fish has not been adequately studied to date. Thus, an acute exposure (96 h) to triazophos at different concentrations of 0.06, 0.3 and 1.5 mg/L (corresponding to 1/50th, 1/10th and 1/2th of 96 h-LC50, respectively), was conducted to investigate the triazophos-induced oxidative stress in adult zebrafish (Danio rerio). The results showed that the time- and dose-dependent induction of oxidative stress except for the ROS level in liver after 24 and 48 h exposure, as indicated by increased reactive oxygen species (ROS), malondialdehyde (MDA) level and a compromised antioxidant defense system, including increased superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione (GSH) content as well as the increased at first and decreased afterwards genes expression (Sod1, Sod2, Cat and Gpx) in brain. Simultaneously, ROS and MDA showed an increased trend, SOD activity, CAT activity and GSH content showed a trend of increasing at 24 h and decreasing at 48 h, and then increasing at 96 h and Sod1, Sod2, Cat and Gpx gene showed decreasing at first and then increasing in liver tissue. The present study concluded that the damage of the antioxidant system by triazophos induced oxidative stress in the brain and liver of zebrafish with concomitant lipid peroxidation, which is an important mechanism underlying the triazophos-induced acute toxicity.
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页数:9
相关论文
共 44 条
[1]  
[Anonymous], 1984, METHOD ENZYMOL
[2]  
[Anonymous], MODERN AGROCHEMICALS
[3]   Sublethal effects induced by captopril on Cyprinus carpio as determined by oxidative stress biomarkers [J].
Azalea Cortes-Diaz, Maria Julieta ;
Rodriguez-Flores, Juana ;
Castaneda-Penalvo, Gregorio ;
Galar-Martinez, Marcela ;
Islas-Flores, Hariz ;
Dublan-Garcia, Octavio ;
Manuel Gomez-Olivan, Leobardo .
SCIENCE OF THE TOTAL ENVIRONMENT, 2017, 605 :811-823
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Pesticide monitoring and ecotoxicological risk assessment in surface water bodies and sediments of a tropical agro-ecosystem [J].
Carazo-Rojas, Elizabeth ;
Perez-Rojas, Greivin ;
Perez-Villanueva, Marta ;
Chinchilla-Soto, Cristina ;
Chin-Pampillo, Juan Salvador ;
Aguilar-Mora, Paula ;
Alpizar-Marin, Melvin ;
Masis-Mora, Mario ;
Rodriguez-Rodriguez, Carlos E. ;
Vryzas, Zisis .
ENVIRONMENTAL POLLUTION, 2018, 241 :800-809
[6]   Effects of high temperature on biochemical parameters, oxidative stress, DNA damage and apoptosis of pufferfish (Takifugu obscurus) [J].
Cheng, Chang-Hong ;
Guo, Zhi-Xun ;
Luo, Sheng-Wei ;
Wang, An-Li .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2018, 150 :190-198
[7]   Oxidative stress response and gene expression with acute copper exposure in zebrafish (Danio rerio) [J].
Craig, Paul M. ;
Wood, Chris M. ;
McClelland, Grant B. .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2007, 293 (05) :R1882-R1892
[8]   A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].
ELLMAN, GL ;
COURTNEY, KD ;
ANDRES, V ;
FEATHERSTONE, RM .
BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&
[9]   INHIBITION OF NITRITE FORMATION FROM HYDROXYLAMMONIUM-CHLORIDE - SIMPLE ASSAY FOR SUPEROXIDE-DISMUTASE [J].
ELSTNER, EF ;
HEUPEL, A .
ANALYTICAL BIOCHEMISTRY, 1976, 70 (02) :616-620
[10]  
ISO, 1996, WAT QUAL DET AC LETH