Resveratrol prevents high glucose-induced epithelial-mesenchymal transition in renal tubular epithelial cells by inhibiting NADPH oxidase/ROS/ERK pathway

被引：77

作者：

He, Ting ^{[1
,2
]}

Guan, Xu ^{[1
,2
]}

Wang, Song ^{[3
]}

Xiao, Tangli ^{[1
,2
]}

Yang, Ke ^{[1
,2
]}

Xu, Xinli ^{[1
,2
]}

Wang, Junping ^{[3
]}

Zhao, Jinghong ^{[1
,2
]}

机构：

[1] Third Mil Med Univ, Xinqiao Hosp, Inst Nephrol Chongqing, Dept Nephrol, Chongqing 400037, Peoples R China

[2] Third Mil Med Univ, Xinqiao Hosp, Kidney Ctr PLA, Chongqing 400037, Peoples R China

[3] Third Mil Med Univ, Inst Combined Injury PLA, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2015年 / 402卷 / 0C期

基金：

中国国家自然科学基金;

关键词：

Resveratrol; Epithelial-mesenchymal transition; Diabetic nephropathy; Reactive oxygen species; Extracellular signal-regulated kinase 1/2; PROTEIN-KINASE-C; OXIDATIVE STRESS; KIDNEY-DISEASE; INDUCED APOPTOSIS; ANGIOTENSIN-II; OXIDASE; ACTIVATION; ROS; SIRT1; ALDOSTERONE;

D O I：

10.1016/j.mce.2014.12.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Resveratrol (RSV) is reported to have renoprotective activity against diabetic nephropathy, while the mechanisms underlying its function have not been fully elucidated. In this study, we investigate the effect and related mechanism of RSV against high glucose-induced epithelial to mesenchymal transition (EMT) in human tubular epithelial cells (HK-2). A typical EMT is induced by high glucose in HK-2 cells, accompanied by increased levels of reactive oxygen species (ROS). RSV exhibits a strong ability to inhibit high glucose-induced EMT by decreasing intracellular ROS levels via down-regulation of NADPH oxidase subunits NOX1 and NOX4. The activation of extracellular signal-regulated kinase (ERK1/2) is found to be involved in high glucose-induced EMT in HK-2 cells. RSV, like NADPH oxidase inhibitor diphenyleneiodonium, can block ERK1/2 activation induced by high glucose. Our results demonstrate that RSV is a potent agent against high glucose-induced EMT in renal tubular cells via inhibition of NADPH oxidase/ROS/ERK1/2 pathway. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：13 / 20

页数：8

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