Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population

被引：1

作者：

de Araujo, Leila Garces ^{[1
]}

Prabhu, Anne Sitarama ^{[2
]}

Arraes Pereira, Pedro Antonio ^{[1
]}

da Silva, Gisele Barata ^{[3
]}

机构：

[1] Univ Fed Goias, Dept Biol Geral, BR-74001970 Goiania, Go, Brazil

[2] Embrapa Arroz & Feijao, BR-75375000 Santo Antonio De Goias, Go, Brazil

[3] Univ Fed Vicosa, Dept Fitopatol, BR-36570000 Vicosa, MG, Brazil

来源：

CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2010年 / 10卷 / 01期

关键词：

Anther culture; double haploid; Magnaporthe oryzae; Oryza sativa; molecular markers; BIOTECHNOLOGY;

D O I：

暂无

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A doubled-haploid (DH) population, obtained by anther culture of F-1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09). of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPSI6(2072) ('AGGGGGTTCC') at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC; F1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.

引用

页码：23 / 31

页数：9

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