Study of single-nucleotide polymorphisms by means of electrical conductance measurements

被引：133
作者：
Hihath, J
Xu, BQ
Zhang, PM
Tao, NJ [1 ]
机构：
[1] Arizona State Univ, Dept Elect Engn, Ctr Solid State Elect Res, Tempe, AZ 85287 USA
[2] Arizona State Univ, Ctr Single Mol Biophys, Biodesign Inst, Tempe, AZ 85287 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 47期
关键词：
molecular electronics; scanning tunneling microscope break junction;
D O I：
10.1073/pnas.0505175102
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Understanding the complexities of DNA has been a hallmark of science for over a half century, and one of the important topics in DNA research is recognizing the occurrence of mutations in the base-stack. In this article, we present a study of SNPs by direct-contact electrical measurements to a single DNA duplex. We have used short, 11- and 12-bp dsDNA to investigate the change in conductance that occurs if a single base pair, a single base, or two separate bases in the stack are modified. All measurements are carried out in aqueous solution with the DNA chemically bound to the electrodes. These measurements demonstrate that the presence of a single base pair mismatch can be identified by the conductance of the molecule and can cause a change in the conductance of dsDNA by as much as an order of magnitude, depending on the specific details of the double helix and the single nucleotide polymorphism.
引用
收藏
页码：16979 / 16983
页数：5
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