Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes

被引:28
作者
Zelnickova, Petra [1 ,2 ]
Leva, Lenka [1 ]
Stepanova, Hana [1 ,3 ]
Kovaru, Frantisek [4 ,5 ]
Faldyna, Martin [1 ,2 ]
机构
[1] Vet Res Inst, Dept Immunol, Brno 62100, Czech Republic
[2] Univ Vet & Pharmaceut Sci Brno, Inst Microbiol & Immunol, Brno 61242, Czech Republic
[3] Masaryk Univ, Fac Sci, Inst Expt Biol, CS-61137 Brno, Czech Republic
[4] Acad Sci Czech Republ, Inst Anim Physiol & Genet, Brno, Czech Republic
[5] Univ Vet & Pharmaceut Sci Brno, Inst Physiol, Brno 62100, Czech Republic
关键词
pig; postnatal ontogeny; tumour necrosis factor alpha; interleukin-1; beta; interleukin-8; blood phagocytes;
D O I
10.1016/j.vetimm.2008.04.016
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim of the present study was to determine postnatal ontogeny of proinflammatory cytokines IL-1 beta, IL-8 and TNF-alpha production by in vitro Stimulated porcine blood leukocytes. Four age categories of pigs were chosen. Cytokine production Was determined using intracellular flow cytometry. It Was found that IL-8 and TNF-alpha production by blood monocytes significantly increased during the postnatal period while production of IL-1 beta remained Unchanged. In blood neutrophils, the IL-8 production increased only during the postnatal period, while the levels of TNF-alpha and IL-1 beta were undetectable during the whole postnatal period. Generally, the most intensive changes in cytokine production occurred before weaning. The production of low levels of cytokines by monocytes and neutrophils from young pigs was not caused by a delayed cytokine response because the cytokine production after 8-h Stimulation was lower than that after 4-h stimulation in all age categories. The ontogenetical changes showed the same trends when two different stimulators (LPS, heat-inactivated E. coli) were used, suggesting that the ontogenetical changes are not caused by a simple defect in one signalling pathway, bill it is probably a more complex process. No differences in cytokine production between the whole blood and the isolated cells supplemented with newborn or adult serum were found. Thus the ability of newborn monocytes and neutrophils to produce proinflammatory cytokines was not decreased due to the influence of composition of the microenvironment, where the cells were present. In conclusion, the ability of porcine blood leukocytes to produce cytokines develops during postnatal life. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:367 / 378
页数:12
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