Rapid laboratory diagnosis of Wilson's disease: One-step simultaneous detection of exchangeable copper and ceruloplasmin in serum based on nanotechnology

被引:7
作者
Xiong, Yamin [1 ,2 ]
Zhou, Lihong [1 ,2 ]
Wang, Hui [1 ,2 ]
Li, Huiling [3 ]
Hu, Yue [1 ,2 ]
Meng, Peijun [1 ,4 ]
Zhang, Xueying [1 ,2 ]
Huang, Peili [1 ,2 ]
机构
[1] Capital Med Univ, Sch Publ Hlth, Dept Toxicol & Sanit Chem, 10 Xitoutiao, Beijing 100069, Peoples R China
[2] Beijing Key Lab Environm Toxicol, Beijing 100069, Peoples R China
[3] Capital Med Univ, Beijing Chao Yang Hosp, Dept Occupat Med & Clin Toxicol, Beijing 100020, Peoples R China
[4] Inner Mongolia Univ Sci & Technol, Baotou Med Coll, Sch Publ Hlth, Baotou 014060, Peoples R China
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 281卷
基金
北京市自然科学基金; 中国国家自然科学基金;
关键词
Wilson's disease; Exchangeable copper; Ceruloplasmin; One-step simultaneous detection; Magnetic nanoparticles; Quantum dots; SIGNAL AMPLIFICATION STRATEGY; MAGNETIC FE3O4 NANOPARTICLES; SENSITIVE DETECTION; PROTEIN BIOMARKERS; FLUORESCENCE; ASSAY; DOTS;
D O I
10.1016/j.snb.2018.11.012
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Monitoring the level of exchangeable copper (CuEXC) and ceruloplasmin (Cp) in serum is of great significance for Wilson's disease (WD). In this work, a facile one-step competitive immunoassay for rapid and simultaneous detection of CuEXC and Cp has been developed based on antigens coated magnetic nanoparticles (immuno-MNPs) and antibodies functionalized quantum dots (QDs-Abs). Just by mixing immuno-MNPs, with diluted serum and QDs-Abs together, the antigens on immuno-MNPs compete with the target in serum and bind to QDs-Abs, thereby causing the changes in fluorescence intensity of QDs. Under selected conditions, this one-step method exhibited excellent precision and selectivity with the measured limit of detection of 0.2 nM and 7.0 mu g/L for CuEXC and Cp, respectively. Most importantly, this method was successfully applied to detect CuEXC and Cp without the usual time-consuming pretreatment. The results showed no significant difference (P>0.01) from those of ICP-AES and ELISA; moreover, CuEXC significantly decreased to a normal level after the treatment of preliminarily diagnosed WD patients, while the changes of Cp level is not significant (P>0.01). Therefore, the proposed one-step competitive immunoassay has great potential applications in the rapid laboratory diagnosis of WD with distinct advantages over the existing methods in terms of multi-target, accuracy, facile, fast and cost-effectiveness.
引用
收藏
页码:713 / 719
页数:7
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