Lactobacillus nasuensis sp nov., a lactic acid bacterium isolated from silage, and emended description of the genus Lactobacillus

被引:35
作者
Cai, Yimin [1 ]
Pang, Huili [2 ]
Kitahara, Maki [3 ]
Ohkuma, Moriya [3 ]
机构
[1] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan
[2] Zhengzhou Univ, Zhengzhou 450052, Henan, Peoples R China
[3] RIKEN BioResource Ctr, Wako, Saitama 3510198, Japan
关键词
DEOXYRIBONUCLEIC-ACID; FORAGE CROPS; PERFORMANCE; MICROFLORA; SOURDOUGH;
D O I
10.1099/ijs.0.031781-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two strains of lactic acid bacteria, designated SU 18(T) and SU 83, were isolated from silage prepared with Sudan grass [Sorghum sudanense (Piper) Stapf.]. The isolates were Gram-stain-positive, catalase-negative, facultatively anaerobic rods that did not produce gas from glucose. The isolates exhibited >= 93.5% DNA-DNA relatedness to each other and shared the same phenotypic characteristics, which indicated that they belonged to a single species. The DNA G + C content was 58.5-59.2 mol%. On the basis of 16S rRNA gene sequence analysis, the isolates were placed in the genus Lactobacillus. Their closest phylogenetic neighbours were Lactobacillus manihotivorans JCM 12514(T) and Lactobacillus camelliae JCM 13995(T) (95.9 and 96.8 % 16S rRNA gene sequence similarity, respectively, with strain SU 18(T)). Ribotyping revealed that strain SU 18(T). was well separated from L. manihotivorans JCM 12514(T) and L. camelliae JCM 13995(T). Strain SU 18(T) exhibited <= 23.7% DNA-DNA relatedness with its closest phylogenetic neighbours. The isolates represent a novel species in the genus Lactobacillus, for which the name Lactobacillus nasuensis sp. nov. is proposed. The type strain is SU 18(T) (=JCM 17158(T) =CGMCC 1.10801(T)). The description of the genus Lactobacillus is also amended.
引用
收藏
页码:1140 / 1144
页数:5
相关论文
共 22 条
[1]  
Beijerinck M.W., 1901, Archives Neerlandaises des Sciences Exactes et Naturelles (Section 2), V6, P212
[2]   Advantage of using inosine at the 3′ termini of 16S rRNA gene universal primers for the study of microbial diversity [J].
Ben-Dov, Eitan ;
Shapiro, Orr H. ;
Siboni, Nachshon ;
Kushmaro, Ariel .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (11) :6902-6906
[3]   Lactococcus fujiensis sp nov., a lactic acid bacterium isolated from vegetable matter [J].
Cai, Yimin ;
Yang, Jinsong ;
Pang, Huili ;
Kitahara, Maki .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011, 61 :1590-1594
[4]  
Cai YM, 1998, APPL ENVIRON MICROB, V64, P2982
[5]   Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation [J].
Cai, YM .
JOURNAL OF DAIRY SCIENCE, 1999, 82 (11) :2466-2471
[6]   Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis [J].
Ennahar, S ;
Cai, YM ;
Fujita, Y .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (01) :444-451
[7]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229
[8]  
Kandler O., 1986, BERGEYS MANUAL SYSTE, VII, P1209
[9]   STOCHASTIC-MODEL FOR ESTIMATION OF MUTATIONAL DISTANCE BETWEEN HOMOLOGOUS PROTEINS [J].
KIMURA, M ;
OHTA, T .
JOURNAL OF MOLECULAR EVOLUTION, 1972, 2 (01) :87-90
[10]  
Kozaki M., 1992, Experimental Manual for Lactic Acid Bacteria, P29