Injectable and bioresorbable calcium phosphate delivery system with gentamicin sulphate for treatment of bone diseases: in vitro study

In this present research, calcium phosphate cement (CPC) paste was prepared by combining cement liquids comprised of 4 wt-% disodium hydrogen orthophosphate with cement powders that consisted of beta-tricalcium phosphate and monocalcium phosphate monohydrate. In the in vitro study, the release of gentamicin sulphate (GS) from standard cylindrical samples of the prepared CPC immersion in phosphate buffered saline solution was measured by high performance liquid chromatography test over a period of time. In addition, as a representative for biomedical properties, setting time, injectability and compressive strength were measured in order to detect changes when mixing CPC with GS. In addition, the pH values of the phosphate buffered saline solution containing samples descended gradually until reaching equilibrium pH. Overall, the results from in vitro release study showed that it reached its maximum level, which was 35% of the initial value of the GS on day 15, suggesting no irreversible binding occurred between the cement paste and the antibiotic.