Microbial biosensor based on whole cell of Pseudomonas sp for online measurement of p-nitrophenol

被引：38

作者：

Banik, R. M. ^{[2
]}

Mayank ^{[2
]}

Prakash, Rajiv ^{[1
]}

Upadhyay, S. N. ^{[3
]}

机构：

[1] Banaras Hindu Univ, Inst Technol, Sch Mat Sci & Technol, Varanasi 221005, Uttar Pradesh, India

[2] Banaras Hindu Univ, Inst Technol, Sch Biochem Engn, Varanasi 221005, Uttar Pradesh, India

[3] Banaras Hindu Univ, Inst Technol, Dept Chem Engn & Technol, Varanasi 221005, Uttar Pradesh, India

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 131卷 / 01期

关键词：

amperometric sensor; microbial biosensor; p-nitrophenol; oxygen electrode; Pseudomonas sp;

D O I：

10.1016/j.snb.2007.11.022

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Whole cells of Pseudomonas sp. were employed as the primary sensing element of a microbial biosensor suitable for online estimation of p-nitrophenol based on a Clark type oxygen electrode. In this process p-nitrophenol adapted Pseudomonas cells were immobilized on the polycarbonate membrane having pore size of 0.4 mu m and placed on the tip of Clark type dissolved oxygen (DO) electrode to measure the change in oxygen concentration due to degradation of p-nitrophenol and consumption of oxygen by the Pseudomonas cells. The present study was carried out, keeping in view the possible use of the biosensor for field and online measurements of p-nitrophenol. It was tried to overcome the major limitations of the microbial biosensors viz. slow response, fixed temperature and requirement of a buffered solution of fixed strength and pH by developing a p-nitrophenol adapted Pseudomonas sp. microbial biosensor in a wide range of temperature and pH. The biosensor is suitable for quantification of p-nitrophenol in the concentration range of 10 - 50 mu M with the varying pH (7.5 - 8.5) and temperature (25 - 40 degrees C) in short response time (7.0 min). (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：295 / 300

页数：6

共 32 条

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