The determination of the extracellular concentration of brain glutamate using quantitative microdialysis

被引：59

作者：

Miele, M ^{[1
]}

Berners, M ^{[1
]}

Boutelle, MG ^{[1
]}

Kusakabe, H ^{[1
]}

Fillenz, M ^{[1
]}

机构：

[1] UNIV OXFORD,PHYSIOL LAB,OXFORD OX1 3PT,ENGLAND

来源：

BRAIN RESEARCH | 1996年 / 707卷 / 01期

关键词：

quantitative microdialysis; glutamate; rat striatum; enzyme assay; extracellular concentration; unanesthetized;

D O I：

10.1016/0006-8993(95)01371-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Quantitative microdialysis with two enzyme-based assays was used to determine the extracellular concentration of glutamate in the striatum of freely moving rats. From the difference between infused and dialysate glutamate a value of 3.0 +/- 0.6 mu M for the extracellular glutamate concentration was computed by regression analysis. The in vivo recovery, derived from the slope of the regression line, was 50%.

引用

页码：131 / 133

页数：3

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