SIREs: searching for iron-responsive elements

被引：69

作者：

Campillos, Monica ^{[1
]}

Cases, Ildefonso ^{[2
]}

Hentze, Matthias W. ^{[1
]}

Sanchez, Mayka ^{[1
,2
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

[2] IMPPC, Barcelona 08916, Spain

来源：

NUCLEIC ACIDS RESEARCH | 2010年 / 38卷

关键词：

FERRITIN MESSENGER-RNA; REGULATORY PROTEINS; TRANSLATIONAL REGULATION; EXPRESSION; REGION; IDENTIFICATION; TRANSPORTER; PREVENTS; SEQUENCE; MUTATION;

D O I：

10.1093/nar/gkq371

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The iron regulatory protein/iron-responsive element regulatory system plays a crucial role in the post-transcriptional regulation of gene expression and its disruption results in human disease. IREs are cis-acting regulatory motifs present in mRNAs that encode proteins involved in iron metabolism. They function as binding sites for two related trans-acting factors, namely the IRP-1 and -2. Among cis-acting RNA regulatory elements, the IRE is one of the best characterized. It is defined by a combination of RNA sequence and structure. However, currently available programs to predict IREs do not show a satisfactory level of sensitivity and fail to detect some of the functional IREs. Here, we report an improved software for the prediction of IREs implemented as a user-friendly web server tool. The SIREs web server uses a simple data input interface and provides structure analysis, predicted RNA folds, folding energy data and an overall quality flag based on properties of well characterized IREs. Results are reported in a tabular format and as a schematic visual representation that highlights important features of the IRE. The SIREs (Search for iron-responsive elements) web server is freely available on the web at http://ccbg.imppc.org/sires/index.html

引用

页码：W360 / W367

页数：8

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