Expression of LOX-1 in human mesangial cells is increased by Ox-LDL and IL-1β treatment

被引：3
作者：
Deng, Yinghui [1 ]
Lin, Na [1 ]
Wu, Leiyun [1 ]
Jia, Qaing [1 ]
Liu, Hua [1 ]
机构：
[1] Capital Med Univ, Xuanwu Hosp, Dept Nephrol, 45 Changchun St, Beijing 100053, Peoples R China
来源：
EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2017年 / 14卷 / 04期
关键词：
oxidized low-density lipoprotein; human mesangial cell; lectin-like oxidized LDL receptor 1; interleukin; 1; beta; OXIDIZED LDL; LIPID-ACCUMULATION; RECEPTOR; GLOMERULOSCLEROSIS; ATHEROSCLEROSIS; APOPTOSIS; PATHWAY;
D O I：
10.3892/etm.2017.4950
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The present study aimed to determine whether the expression of lectin-like oxidized LDL receptor 1 (LOX-1) could be induced by oxidized low-density lipoprotein (Ox-LDL) and interleukin 1 beta (IL-1 beta) in human mesangial cells (HMCs). Oil Red O staining was used to observe the uptake of Ox-LDL by HMCs stimulated with IL-1 beta, and reverse transcription-quantitative polymerase chain reaction analysis and western blotting were used to examine the expression of LOX-1 in HMC following Ox-LDL and IL-1 beta treatment. Uptake of Ox-LDL by HMCs was upregulated upon stimulation with IL-1 beta. Furthermore, Ox-LDL (10-40 mu g/ml) treatment induced LOX-1 mRNA and protein expression in a dose-dependent manner. In addition, when HMCs were treated with IL-1 beta and Ox-LDL, the expression of LOX-1 was enhanced further. These results indicated that inhibiting LOX-1 expression or inhibiting the Ox-LDL/LOX-1 signaling axis may be a potential novel method for treating renal disease.
引用
收藏
页码：3632 / 3636
页数：5
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