Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane

被引：49
|
作者：
Huang, WM [1 ]
Jia, JB [1 ]
Zhang, ZL [1 ]
Han, XJ [1 ]
Tang, JL [1 ]
Wang, JG [1 ]
Dong, SJ [1 ]
Wang, EK [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
来源：
BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 10期
关键词：
microperoxidase-11; lipid membrane; didodecyldimethylammonium bromide; biosensor;
D O I：
10.1016/S0956-5663(03)00070-8
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H2O2 was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H2O2 without electron mediator or promoter, at a potential of +0.10 V versus Ag \ AgCl. A linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) M (S/N = 3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：1225 / 1230
页数：6
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