Hydrogen peroxide biosensor based on microperoxidase-11 entrapped in lipid membrane

被引：49

作者：

Huang, WM ^{[1
]}

Jia, JB ^{[1
]}

Zhang, ZL ^{[1
]}

Han, XJ ^{[1
]}

Tang, JL ^{[1
]}

Wang, JG ^{[1
]}

Dong, SJ ^{[1
]}

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 10期

基金：

中国国家自然科学基金;

关键词：

microperoxidase-11; lipid membrane; didodecyldimethylammonium bromide; biosensor;

D O I：

10.1016/S0956-5663(03)00070-8

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A highly catalytic activity microperoxidase-11 (MP-11) biosensor for H2O2 was developed to immobilizing the heme peptide in didodecyldimethylammonium bromide (DDAB) lipid membrane. The enzyme electrode thus obtained responded to H2O2 without electron mediator or promoter, at a potential of +0.10 V versus Ag \ AgCl. A linear calibration curve is obtained over the range from 2.0 x 10(-5) to 2.4 x 10(-3) M. The biosensor responds to hydrogen peroxide in 15 s and has a detection limit of 8 x 10(-7) M (S/N = 3) Providing a natural environment with lipid membrane for protein immobilization and maintenance of protein functions is a suitable option for the design of biosensors. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：1225 / 1230

页数：6

共 28 条

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