Protective Effects of Loquat (Eriobotrya japonica) Leaves against Ethanol-induced Toxicity in HepG2 Cells Transfected with CYP2E1

被引：19

作者：

Bae, Donghyuck ^{[1
]}

You, Yanghee ^{[2
]}

Yoon, Ho-Geun ^{[3
]}

Kim, Kyungmi ^{[4
]}

Lee, Yoo-Hyun ^{[5
]}

Kim, Yongjae ^{[1
,6
]}

Baek, Humyoung ^{[6
]}

Kim, Sunoh ^{[7
]}

Lee, Jeongmin ^{[8
]}

Jun, Woojin ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Dept Food & Nutr, Kwangju 550757, South Korea

[2] Chonnam Natl Univ, Human Ecol Res Inst, Kwangju 550757, South Korea

[3] Yonsei Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 120752, South Korea

[4] Korea Bio Polytech Coll, Dept Biofood Anal, Nonsan 320905, Chungnam, South Korea

[5] Univ Suwon, Dept Food & Nutr, Suwon 445743, Gyeonggi, South Korea

[6] Korea INS Pharm Res Inst, Hwasun 519882, Jeonnam, South Korea

[7] Jeonnam Inst Nat Resources Res, Jangheung 529851, Jeonnam, South Korea

[8] Kyung Hee Univ, Res Inst Med Nutr, Yongin 446701, Gyeonggi, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2010年 / 19卷 / 04期

关键词：

loquat; HepG2/2E1; hepatoprotection; oxidative stress; antioxidation;

D O I：

10.1007/s10068-010-0154-3

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hepatoprotective effects of loquat (Eriobotrya japonica) leaves were investigated in HepG2 cells over-expressing CYP2E1. When compared to cells treated with 200 mM ethanol alone, a concentration-dependent increase in cell viability was observed in the cells pretreated with 40 and 80 mu g/mL of 5% ethanol extract (EJE) of loquat leaves (23 and 36%, respectively). Also, pretreatment with EJE lead to a decrease in intracellular reactive oxygen species formation and an increase in hepatic antioxidant activity. These results suggest that EJE attenuates oxidative stress by improving antioxidative potentials, which contribute to this herb's protective profile against ethanol-induced toxicity in vitro.

引用

页码：1093 / 1096

页数：4

共 17 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2]

Akerboom T P, 1981, Methods Enzymol, V77, P373

[3] Oxidative mechanisms in the pathogenesis of alcoholic liver disease [J].

Albano, Emanuele .

MOLECULAR ASPECTS OF MEDICINE, 2008, 29 (1-2) :9-16

[4] Anti-inflammatory and antitumor-promoting effects of the triterpene acids from the leaves of Eriobotrya japonica [J].

Banno, N ;

Akihisa, T ;

Tokuda, H ;

Yasukawa, K ;

Taguchi, Y ;

Akazawa, H ;

Ukiya, M ;

Kimura, Y ;

Suzuki, T ;

Nishino, H .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2005, 28 (10) :1995-1999

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6]

CARLBERG I, 1975, J BIOL CHEM, V250, P5475

[7] Microwave-assisted extraction followed by capillary electrophoresis-amperometric detection for the determination of antioxidant constituents in Folium Eriobotryae [J].

Chen, Zhi ;

Zhang, Luyan ;

Chen, Gang .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1193 (1-2) :178-181

[8] CHANGES IN THE LEVELS OF ENZYMES WHICH MODULATE THE ANTIOXIDANT BALANCE OCCUR DURING AGING AND CORRELATE WITH CELLULAR-DAMAGE [J].

CRISTIANO, F ;

DEHAAN, JB ;

IANNELLO, RC ;

KOLA, I .

MECHANISMS OF AGEING AND DEVELOPMENT, 1995, 80 (02) :93-105

[9]

Habig W H, 1981, Methods Enzymol, V77, P398

[10] Mechanisms of liver injury. III. Role of glutathione redox status in liver injury [J].

Han, Derick ;

Hanawa, Naoko ;

Saberi, Behnam ;

Kaplowitz, Neil .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2006, 291 (01) :G1-G7

← 1 2 →